Heparin-Binding Epidermal Growth Factor–Like Growth Factor, Collateral Vessel Development, and Angiogenesis in Skeletal Muscle Ischemia
| Autor: | Susan W. Sunnarborg, Hua Zhang, David C. Lee, Dan Chalothorn, Scott M. Moore, James E. Faber |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Angiogenesis medicine.medical_treatment Ischemia Neovascularization Physiologic Cardiomegaly Femoral artery Fibroblast growth factor Mice Gastrocnemius muscle Epidermal growth factor Internal medicine medicine.artery medicine Animals Gracilis muscle Phosphorylation Muscle Skeletal Ligation Epidermal Growth Factor business.industry Growth factor Anatomy medicine.disease Mice Mutant Strains Hindlimb ErbB Receptors Femoral Artery Phenotype Endocrinology Intercellular Signaling Peptides and Proteins Cardiology and Cardiovascular Medicine business Heparin-binding EGF-like Growth Factor |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 25:1884-1890 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000175761.59602.16 |
| Popis: | Objective— Heparin-binding epidermal growth factor–like growth factor (HB-EGF) is a potent mitogen for smooth muscle cells and has been implicated in atherosclerosis, tissue regeneration after ischemia, vascular development, and tumor angiogenesis. We examined the hypothesis that HB-EGF participates in angiogenesis and collateral growth in ischemia. Methods and Results— During 3 weeks after femoral artery ligation, no attenuation occurred in recovery of hindlimb perfusion or distal saphenous artery flow in HB-EGF–null (HB-EGF −/− ) versus wild-type mice. Lumen diameters of remodeled collaterals in gracilis muscle were similar by morphometry (87±8 versus 94±6 μm) and angiography, although medial thickening was reduced. Gastrocnemius muscle underwent comparable angiogenesis (41% and 33% increase in capillary-to-muscle fiber ratio). Renal renin mRNA, arterial pressure, and heart rate during anesthesia or conscious unrestrained conditions were similar between groups. These latter findings validate comparisons of perfusion data and also suggest that differences in arterial pressure and/or renin–angiotensin activity are not masking an otherwise inhibitory effect of HB-EGF absence. Four days after ligation, EGF receptor phosphorylation increased in muscle by 104% in wild-type but by only 30% in HB-EGF −/− mice. This argues against compensation by other EGF receptor ligands. Conclusion— Our results suggest that HB-EGF is not required for arteriogenesis or angiogenesis in hindlimb ischemia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|