Effect of Patient-Specific Preanalytic Variables on CSF Aβ1–42 Concentrations Measured on an Automated Chemiluminescent Platform
Autor: | Karen Bandeen-Roche, Amanda Calabro, Amanze Orusakwe, Rianne Esquivel, Kristina Khingelova, Jacqueline Darrow, Marilyn S. Albert, Abhay Moghekar, Aruna Rao, Christopher Traynham, Sara Gannon, Seema Gulyani |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Blood contamination Physiology tau Proteins Article law.invention 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Alzheimer Disease law medicine Humans Dementia Cognitive Dysfunction Cognitive impairment Chemiluminescence Amyloid beta-Peptides business.industry General Medicine Patient specific medicine.disease Peptide Fragments 030104 developmental biology Clinical diagnosis Alzheimer's disease business 030217 neurology & neurosurgery |
Zdroj: | J Appl Lab Med |
ISSN: | 2475-7241 2576-9456 |
Popis: | Background Cerebrospinal fluid (CSF) biomarkers are increasingly used to confirm the accuracy of a clinical diagnosis of mild cognitive impairment or dementia due to Alzheimer disease (AD). Recent evidence suggests that fully automated assays reduce the impact of some preanalytical factors on the variability of these measures. This study evaluated the effect of several preanalytical variables common in clinical settings on the variability of CSF β-amyloid 1–42 (Aβ1–42) concentrations. Methods Aβ1–42 concentrations were measured using the LUMIPULSE G1200 from both freshly collected and frozen CSF samples. Preanalytic variables examined were: (1) patient fasting prior to CSF collection, (2) blood contamination of specimens, and (3) aliquoting specimens sequentially over the course of collection (i.e., CSF gradients). Results Patient fasting did not significantly affect CSF Aβ1–42 levels. While assessing gradient effects, Aβ1–42 concentrations remained stable within the first 5 1-mL aliquots. However, there is evidence of a gradient effect toward higher concentrations over successive aliquots. Aβ1–42 levels were stable when fresh CSF samples were spiked with up to 2.5% of blood. However, in frozen CSF samples, even 0.25% blood contamination significantly decreased Aβ1–42 concentrations. Conclusions The preanalytical variables examined here do not have significant effects on Aβ1–42 concentrations if fresh samples are processed within 2 h. However, a gradient effect can be observed on Aβ1–42 concentrations after the first 5 mL of collection and blood contamination has a significant impact on Aβ1–42 concentrations once specimens have been frozen. |
Databáze: | OpenAIRE |
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