Quantification of Interferon Signaling in Avian Cells

Autor:	Maria Forlenza, Joeri Kint
Rok vydání:	2015
Předmět:	Ifn response Celbiologie en Immunologie Biology Chicken Virology Real-time quantitative PCR Real-time polymerase chain reaction Cell Biology and Immunology Interferon Gene expression WIAS medicine Bioassay medicine.drug
Zdroj:	Coronaviruses ISBN: 9781493924370 Coronaviruses: Methods and Protocols Coronaviruses: Methods and Protocols. Springer
DOI:	10.1007/978-1-4939-2438-7_21
Popis:	Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7364421eeacb184b2a17c0b53dd0fdcc https://doi.org/10.1007/978-1-4939-2438-7_21 Zobrazit plný text záznamu