Role of the 75-kDa tumor necrosis factor receptor: inhibition of early hematopoiesis
| Autor: | Lars Slørdal, Frede W. Jacobsen, Erlend B. Smeland, O. P. Veiby, David V. Goeddel, Mike Rothe, Leiv S. Rusten, Sten Eirik W. Jacobsen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Macrophage colony-stimulating factor
Cellular differentiation Bone Marrow Cells Biology Receptors Tumor Necrosis Factor Colony-Forming Units Assay Mice Radioligand Assay Cell surface receptor Granulocyte Colony-Stimulating Factor medicine Animals Humans Receptor Cells Cultured Interleukin 3 Multidisciplinary Tumor Necrosis Factor-alpha Macrophage Colony-Stimulating Factor Granulocyte-Macrophage Colony-Stimulating Factor Cell Differentiation Hematopoietic Stem Cells Molecular biology Recombinant Proteins Hematopoiesis Mice Inbred C57BL Molecular Weight Haematopoiesis Granulocyte macrophage colony-stimulating factor Tumor necrosis factor alpha Cell Division Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 91:10695-10699 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Biological effects of tumor necrosis factor alpha (TNF-alpha) are mediated through two cell surface receptors, the 55-kDa TNF receptor and the 75-kDa TNF receptor. The present study investigated the relative roles of the two TNF receptors in normal hematopoiesis. Using agonists (antibodies) specific for the 55- and 75-kDa TNF receptors, we demonstrate differential roles of the two TNF receptors in hematopoiesis in that only the 55-kDa TNF receptor mediates antiproliferative effects of TNF-alpha on mature Lin- hematopoietic progenitor cells responding to granulocyte colony-stimulating factor or interleukin 3 alone. In contrast, the 75-kDa TNF receptor is essential in mediating inhibition of primitive Lin-Sca-1+ high-proliferative-potential colony-forming cells and inhibition of the total number of proliferative clones of individually cultured Lin-Sca-1+Rh123lo and Lin-Sca-1+Rh123hi cells. |
| Databáze: | OpenAIRE |
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