In Vivo Effects of Quercetin in Association with Moderate Exercise Training in Improving Streptozotocin-Induced Aortic Tissue Injuries
| Autor: | Simona Clichici, Ramona Simedrea, Irina Camelia Chis, Andrei Coseriu, Adrian Oros, Andras Nagy |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Blood Glucose
Male Drug Evaluation Preclinical Pharmaceutical Science Nitric Oxide Synthase Type II Aorta Thoracic medicine.disease_cause endothelial dysfunction diabetes mellitus quercetin exercise training Antioxidants Analytical Chemistry chemistry.chemical_compound Drug Discovery Thoracic aorta Endothelial dysfunction biology Malondialdehyde Exercise Therapy Nitric oxide synthase Cholesterol Chemistry (miscellaneous) Molecular Medicine medicine.drug medicine.medical_specialty Diabetic angiopathy Nitric Oxide Streptozocin Article Diabetes Mellitus Experimental lcsh:QD241-441 lcsh:Organic chemistry Internal medicine medicine.artery Diabetes mellitus medicine Animals Physical and Theoretical Chemistry Rats Wistar Swimming Triglycerides business.industry Organic Chemistry medicine.disease Streptozotocin Oxidative Stress Endocrinology chemistry biology.protein business Oxidative stress Diabetic Angiopathies |
| Zdroj: | Molecules Volume 20 Issue 12 Pages 21770-21786 Molecules; Volume 20; Issue 12; Pages: 21770-21786 Molecules, Vol 20, Iss 12, Pp 21770-21786 (2015) |
| ISSN: | 1420-3049 |
| Popis: | Background: Diabetes mellitus (DM) is a chronic endocrine-metabolic disorder associated with endothelial dysfunction. Hyperglycemia, dyslipidemia and abnormal nitric oxide-mediated vasodilatation are the major causal factors in the development of endothelial dysfunction in DM. The prevention of endothelial dysfunction may be a first target against the appearance of atherosclerosis and cardiovascular diseases. We have investigated the synergistic protective effects of quercetin administration and moderate exercise training on thoracic aorta injuries induced by diabetes. Methods: Diabetic rats that performed exercise training were subjected to a swimming training program (1 h/day, 5 days/week, 4 weeks). The diabetic rats received quercetin (30 mg/kg body weight/day) for 4 weeks. At the end of the study, the thoracic aorta was isolated and divided into two parts one part was immersed in 10% formalin for histopathological evaluations and the other was frozen for the assessment of oxidative stress markers (malondialdehyde, MDA and protein carbonyls groups, PC), the activity of antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT), nitrite plus nitrate (NOx) production and inducible nitric oxide synthase (iNOS) protein expression. Results: Diabetic rats showed significantly increased MDA and PC levels, NOx production and iNOS expression and a reduction of SOD and CAT activity in aortic tissues. A decrease in the levels of oxidative stress markers, NOx production and iNOS expression associated with elevated activity of antioxidant enzymes in the aortic tissue were observed in quercetin-treated diabetic trained rats. Conclusions: These findings suggest that quercetin administration in association with moderate exercise training reduces vascular complications and tissue injuries induced by diabetes in rat aorta by decreasing oxidative stress and restoring NO bioavailability. |
| Databáze: | OpenAIRE |
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