Cytologic and DNA-Cytometric Early Diagnosis of Oral Cancer
| Autor: | Kristiane Knops, Natalja Pomjanski, Stefanie Mathes, Alfred Böcking, Alexander Hemprich, Torsten W. Remmerbach, Horst Weidenbach |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Cytological Techniques lcsh:RC254-282 Cytology medicine Carcinoma Humans lcsh:QH573-671 Oral mucosa oral exfoliative cytology DNA‐image cytometry Aged Image Cytometry Aged 80 and over Mouth neoplasm lcsh:Cytology business.industry Oral cancer Carcinoma in situ Reproducibility of Results Cancer DNA Middle Aged lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Aneuploidy medicine.disease medicine.anatomical_structure cancer screening Carcinoma Squamous Cell diagnostic accuracy Female Mouth Neoplasms Other business Cytometry Algorithms DNA‐aneuploidy |
| Zdroj: | Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology Analytical Cellular Pathology, Vol 22, Iss 4, Pp 211-221 (2001) |
| ISSN: | 1878-3651 0921-8912 |
| DOI: | 10.1155/2001/807358 |
| Popis: | Objective. The aim of this prospective study was to report on the diagnostic accuracy of conventional oral exfoliative cytology taken from white‐spotted, ulcerated or other suspicious oral lesions in our clinic. In addition we checked DNA‐image cytometry as an adjuvant diagnostic tool. Our hypothesis is that DNA‐aneuploidy is a sensitive and specific marker for the early identification of tumor cells in oral brushings. Study design. 251 cytological diagnoses obtained from exfoliative smears of 181 patients from macroscopically suspicious lesions of the oral mucosa and from clinically seemingly benign oral lesions which were exisiced for establishing histological diagnoses were compared with histological and/or clinical follow‐ups of the respective patients. Additionally nuclear DNA‐contents were measured after Feulgen restaining using a TV image analysis system. Results. Sensitivity of our cytological diagnosis on oral smears for the detection of cancer cells was 94.6%, specificity 99.5%, positive predictive value 98.1% and negative predictive value 98.5%. DNA‐aneuploidy was assumed if abnormal DNA‐stemlines or cells with DNA‐content greater 9c were observed. On this basis the prevalence of DNA‐aneuploidy in smears of oral squamous cell carcinomasin situor invasive carcinomas was 96.4%. Sensitivity of DNA‐aneuploidy in oral smears for the detection of cancer cells was 96.4%, specificity 100%, positive predictive value 100% and negative 99.0%. The combination of both techniques increased the sensivity to 98.2%, specificity to 100%, positive predictive value to 100% and negative to 99.5%. Conclusions. Brush cytology of all visible oral lesions, if they are clinically considered as suspicious for cancer, are an easily practicable, cheap, non‐invasive, painless, safe and accurate screening method for detection of oral precancerous lesions, carcinomain situor invasive squamous cell carcinoma in all stages. We conclude that DNA‐image cytometry is a very sensitive, highly specific and objective adjuvant tool for the early identification of neoplastic epithelial cells in oral smears. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|