Distinct Strains of Propionibacterium acnes Induce Selective Human β-Defensin-2 and Interleukin-8 Expression in Human Keratinocytes Through Toll-Like Receptors
| Autor: | I. Nagy, Andor Pivarcsi, Márta Széll, Edit Urbán, Lajos Kemény, Andrea Koreck |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Chemokine beta-Defensins Cell Survival human β-defensin-2 Receptors Cell Surface keratinocyte Dermatology Biology Biochemistry Microbiology Propionibacterium acnes Gene expression medicine Humans RNA Messenger Interleukin 8 Molecular Biology Cells Cultured Toll-like receptor Membrane Glycoproteins Innate immune system IL-8 Interleukin-8 Cell Biology biology.organism_classification Toll-Like Receptor 2 Random Amplified Polymorphic DNA Technique Toll-like receptors Toll-Like Receptor 4 TLR2 medicine.anatomical_structure Gene Expression Regulation biology.protein Keratinocyte |
| Zdroj: | Journal of Investigative Dermatology. 124:931-938 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/j.0022-202x.2005.23705.x |
| Popis: | Acne is a chronic inflammatory disease of the pilosebaceous follicle. One of the main pathogenetic factors in acne is the increased proliferation of Propionibacterium acnes (P. acnes) in the pilosebaceous unit. We investigated whether direct interaction of P. acnes with keratinocytes might be involved in the inflammation and ductal hypercornification in acne. The capacities of different P. acnes strains to activate the innate immune response and the growth of cultured keratinocytes were investigated. We have found that two clinical isolates of P. acnes significantly induced human beta-defensin-2 (hBD2) messenger RNA (mRNA) expression; in contrast a third clinical isolate and the reference strain (ATCC11828) had no effect on hBD2 mRNA expression. In contrast, all four strains significantly induced the interleukin-8 (IL-8) mRNA expression. The P. acnes-induced increase in hBD2 and IL-8 gene expression could be inhibited by anti-Toll-like receptor 2 (TLR2) and anti-TLR4 neutralizing antibodies, suggesting that P. acnes-induced secretion of soluble factors in keratinocytes are both TLR2 and TLR4 dependent. In addition, the clinical isolate P. acnes (889) was capable of inducing keratinocyte cell growth in vitro. Our findings suggest that P. acnes modulates the antimicrobial peptide and chemokine expression of keratinocytes and thereby contributes to the recruitment of inflammatory cells to the sites of infections. |
| Databáze: | OpenAIRE |
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