High expression of Golgi phosphoprotein-3 is associated with poor survival in patients with hepatocellular carcinoma
| Autor: | Wei Shi, Ai-jun Liao, Jie Yuan, Yuhong Yao, Ying-Qing Li, Bin Zeng, Guangsheng Hu, Yu-Ming Yang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Carcinoma Hepatocellular Blotting Western Kaplan-Meier Estimate Cell Line Metastasis Cell Line Tumor Biomarkers Tumor Humans Medicine RNA Messenger Survival rate Messenger RNA Oncogene Reverse Transcriptase Polymerase Chain Reaction business.industry Liver Neoplasms Hazard ratio Membrane Proteins Hep G2 Cells General Medicine Middle Aged Prognosis medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Hepatocellular carcinoma Multivariate Analysis Cancer research Regression Analysis Biomarker (medicine) Female business |
| Zdroj: | Tumor Biology. 35:8625-8632 |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1007/s13277-014-2105-8 |
| Popis: | Golgi phosphoprotein 3 (GOLPH3) is recently demonstrated to function as an oncogene involved in the development and progression of cancers. However, little is known about GOLPH3 expression and its clinical significance in hepatocellular carcinoma (HCC). The levels of GOLPH3 messenger RNA (mRNA) and protein in HCC cell lines and fresh tissues were determined by quantitative RT-PCR and western blotting. Additionally, the protein expression of GOLPH3 was detected in 167 paraffin-embedded HCC samples by immunohistochemistry. GOLPH3 mRNA and protein was overexpressed in HCC cell lines and tissues than the immortalized normal hepatocyte cell line LO2 and the adjacent nontumorous live tissues, respectively. High GOLPH3 expression was positively correlated with high serum AFP level (P = 0.015) and more tumor recurrence or metastasis (P = 0.010). In addition, HCC patients with high GOLPH3 expression had poorer overall survival (hazard ratio (HR), 1.87; 95 % confidence interval (CI), 1.19-2.94; P = 0.006) and poorer disease-free survival (HR, 1.90; 95 % CI, 1.21-2.98; P = 0.005) than those with low GOLPH3 expression. The cumulative 5-year survival rate was only 35.19 % (95 % CI, 26.18-44.20 %) in the high GOLPH3 expression group, whereas it was 55.93 % (95 % CI, 43.26-68.60 %) in the low GOLPH3 expression group. Furthermore, multivariate Cox regression analysis demonstrated that the expression of GOLPH3, tumor size, and tumor multiplicity were independent prognostic predictors for HCC patients. GOLPH3 was overexpressed in HCC at both the mRNA and protein levels, and high expression of GOLPH3 could be served as a novel and potential prognostic biomarker for HCC patients. |
| Databáze: | OpenAIRE |
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