Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women
| Autor: | Lea Tummeleht, Andres Salumets, Madis Metsis, Tiina Drell, Triin Lillsaar, Gilbert G.G. Donders, Jaak Simm, Ivo Saarma, Anu Aaspõllu, Edda Väin |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Anatomy and Physiology
Female Urology medicine.disease_cause Polymerase Chain Reaction RNA Ribosomal 16S Gardnerella vaginalis Phylogeny Candida 0303 health sciences Multidisciplinary Ecology Bacterial taxonomy Obstetrics and Gynecology Vaginosis Bacterial Biota 3. Good health medicine.anatomical_structure Medical Microbiology Vagina Medicine DNA Intergenic Female Bacterial vaginosis medicine.symptom Research Article Vaginal discharge Adult Estonia Ecological Metrics Adolescent Science Mycology Biology Microbiology 03 medical and health sciences medicine Humans Microbiome 030304 developmental biology 030306 microbiology Bacterial Taxonomy Reproductive System Species Diversity Bacteriology Sequence Analysis DNA medicine.disease Lactobacillus Vaginal Discharge Metagenomics Fungal Classification Asymptomatic Diseases Pyrosequencing Metagenome |
| Zdroj: | PLoS ONE, Vol 8, Iss 1, p e54379 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The application of high-throughput sequencing methods has raised doubt in the concept of the uniform healthy vaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition. As this needs to be analyzed more extensively and there is little straightforward data regarding the vaginal mycobiome of asymptomatic women we aimed to define bacterial and fungal communities in vaginal samples from 494 asymptomatic, reproductive-age Estonian women. The composition of the vaginal microbiota was determined by amplifying bacterial 16S rRNA and fungal internal transcribed spacer-1 (ITS-1) regions and subsequently sequencing them using 454 Life Sciences pyrosequencing. We delineated five major bacterial community groups with distinctive diversity and species composition. Lactobacilli were among the most abundant bacteria in all groups, but also members of genus Gardnerella had high relative abundance in some of the groups. Microbial diversity increased with higher vaginal pH values, and was also higher when a malodorous discharge was present, indicating that some of the women who consider themselves healthy may potentially have asymptomatic bacterial vaginosis (BV). Our study is the first of its kind to analyze the mycobiome that colonizes the healthy vaginal environment using barcoded pyrosequencing technology. We observed 196 fungal operational taxonomic units (OTUs), including 16 OTUs of Candida spp., which is more diverse than previously recognized. However, assessing true fungal diversity was complicated because of the problems regarding the possible air-borne contamination and bioinformatics used for identification of fungal taxons as significant proportion of fungal sequences were assigned to unspecified OTUs. |
| Databáze: | OpenAIRE |
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