Hyphal induction in the human fungal pathogen Candida albicans reveals a characteristic wall protein profile
| Autor: | Henk L. Dekker, Chris G. de Koster, Alice G. Sorgo, Adriaan R. Siliakus, Leo J. de Koning, Stanley Brul, Clemens J. Heilmann, Frans M. Klis |
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| Přispěvatelé: | Molecular Biology and Microbial Food Safety (SILS, FNWI), Mass Spectrometry of Biomacromolecules (SILS, FNWI) |
| Rok vydání: | 2011 |
| Předmět: |
Hyphal growth
0303 health sciences Hypha Proteome 030306 microbiology Hyphae Virulence Biology biology.organism_classification Microbiology Yeast Corpus albicans Culture Media Fungal Proteins 03 medical and health sciences Biochemistry Cell culture Cell Wall Gene Expression Regulation Fungal Candida albicans Spectroscopy Fourier Transform Infrared Humans 030304 developmental biology |
| Zdroj: | Microbiology-SGM, 157(8), 2297-2307. Society for General Microbiology |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | The ability ofCandida albicansto switch from yeast to hyphal growth is essential for its virulence. The walls and especially the covalently attached wall proteins are involved in the primary host–pathogen interactions. Three hyphal induction methods were compared, based on fetal calf serum, the amino sugarN-acetylglucosamine (GlcNAc) and the mammalian cell culture medium Iscove’s modified Dulbecco’s medium (IMDM). GlcNAc and IMDM were preferred, allowing stable hyphal growth over a prolonged period without significant reversion to yeast growth and with high biomass yields. We employed Fourier transform-MS combined with a15N-metabolically labelled reference culture as internal standard for relative quantification of changes in the wall proteome upon hyphal induction. A total of 21 wall proteins were quantified. Our induction methods triggered a similar response characterized by (i) a category of wall proteins showing strongly increased incorporation levels (Als3, Hwp2, Hyr1, Plb5 and Sod5), (ii) another category with strongly decreased levels (Rhd3, Sod4 and Ywp1) and (iii) a third one enriched for carbohydrate-active enzymes (including Cht2, Crh11, Mp65, Pga4, Phr1, Phr2 and Utr2) and showing only a limited response. This is, to our knowledge, the first systematic, quantitative analysis of the changes in the wall proteome ofC. albicansupon hyphal induction. Finally, we propose new wall-protein-derived candidates for vaccine development. |
| Databáze: | OpenAIRE |
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