Utility of Gram Staining for Evaluation of the Quality of Cystic Fibrosis Sputum Samples
| Autor: | Bindu M. Nair, Jane L. Burns, Lynn Stapp, Jill M. Van Dalfsen, Jenny Stapp, Linda Bugni |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Microbiology (medical) medicine.medical_specialty Pathology Gram-negative bacteria Pancreatic disease Adolescent Cystic Fibrosis Gastroenterology Cystic fibrosis law.invention law Internal medicine Gram-Negative Bacteria Humans Medicine Child Bacteriological Techniques Staining and Labeling biology business.industry Respiratory disease Sputum Bacteriology Middle Aged biology.organism_classification medicine.disease Culture Media Pneumonia Gram staining Phenazines Gentian Violet Culture negative medicine.symptom Gram-Negative Bacterial Infections business |
| Zdroj: | Journal of Clinical Microbiology. 40:2791-2794 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The microscopic examination of Gram-stained sputum specimens is very helpful in the evaluation of patients with community-acquired pneumonia and has also been recommended for use in cystic fibrosis (CF) patients. This study was undertaken to evaluate that recommendation. One hundred one sputum samples from CF patients were cultured for gram-negative bacilli and examined by Gram staining for both sputum adequacy (using the quality [Q] score) and bacterial morphology. Subjective evaluation of adequacy was also performed and categorized. Based on Q score evaluation, 41% of the samples would have been rejected despite a subjective appearance of purulence. Only three of these rejected samples were culture negative for gram-negative CF pathogens. Correlation between culture results and quantitative Gram stain examination was also poor. These data suggest that subjective evaluation combined with comprehensive bacteriology is superior to Gram staining in identifying pathogens in CF sputum. |
| Databáze: | OpenAIRE |
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