FOXP3+ regulatory T-cells are abundant in vulvar Paget's disease and are associated with recurrence
| Autor: | Hisham K. Tamimi, Kimberly H. Allison, Rochelle L. Garcia, Barbara A. Goff, William A. Peters, Ron E. Swensen, Elise N. Everett, Ellen S. Pizer, Heidi J. Gray, Joshua Z. Press |
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| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Disease T-Lymphocytes Regulatory Vulvar Paget's Disease Recurrent disease Positive Margins Humans Medicine Aged Vulvar Neoplasms integumentary system business.industry Obstetrics and Gynecology FOXP3 Forkhead Transcription Factors Immunosuppression Immunohistochemistry Paget Disease Extramammary Oncology Female Neoplasm Recurrence Local Positive Surgical Margin business |
| Zdroj: | Gynecologic Oncology. 120:296-299 |
| ISSN: | 0090-8258 |
| DOI: | 10.1016/j.ygyno.2010.10.019 |
| Popis: | Objective. To characterize clinical features of vulvar Paget's disease, and examine the quantity of immunosuppressive regulatory T-cells in vulvar Paget's tissue. Methods. Vulvar Paget's cases from 1992 to 2007 from two institutions were identified by pathology database search. Regulatory T-cells were identified with FOXP3 immunohistochemistry and quantified at the dermal–epidermal junction using image analysis software. Thirteen non-neoplastic inflammatory cases were stained for comparison. Results. Cases included 33 women treated for primary vulvar Paget's, and 7 referred at recurrence. Of the 24 primary cases with greater than 5months follow-up, recurrence was documented in 12/24(50%). Eight women (20%) recurred multiple times, but no recurrences were invasive. Significantly more patients with positive margins developed recurrent disease (82% vs 23%, p =0.01). Secondary neoplasms occurred in 10/40(25%). FOXP3+ cells at the dermal–epidermal junction were quantified in 29 primary and 13 recurrent tissue samples. FOXP3+ cells were absent in surrounding normal vulvar skin. FOXP3+ cells averaged 66/HPF in primary vulvar Paget's and 66/HPF in recurrent Paget's, compared to 22/HPF in non-neoplastic inflammatory cases ( p =0.0003, p =0.001). Primary cases with positive surgical margins had more FOXP3+ cells than those with negative margins (85 vs 49, p =0.01). Recurrent cases with positive margins had more FOXP3+ cells than negative cases (84 vs 33, p =0.06). FOXP3 levels in primary specimens were higher in cases which recurred (78 vs 35, p =0.02). Conclusions. Increased regulatory T-cells may be associated with more extensive cases of vulvar Paget's disease that result in positive surgical margins and are associated with recurrence of disease, suggesting immunosuppression as a key factor. |
| Databáze: | OpenAIRE |
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