Silencing circPVT1 enhances radiosensitivity in non-small cell lung cancer by sponging microRNA-1208
| Autor: | Chongxin Li, Meifang Huang, Zengbo Lv, Bo Hou, Qing Wang, Yongmei He, Huahua Zhou, Tianqian Li, Shengyi Deng, Guangying Zhu |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Lung Neoplasms Transfection 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Cell Line Tumor microRNA Genetics Humans Gene silencing Radiosensitivity neoplasms PI3K/AKT/mTOR pathway Cell Proliferation 030304 developmental biology 0303 health sciences TUNEL assay Chemistry RNA Circular General Medicine Up-Regulation respiratory tract diseases MicroRNAs Oncology Terminal deoxynucleotidyl transferase Apoptosis 030220 oncology & carcinogenesis Cancer research RNA Long Noncoding |
| Zdroj: | Cancer Biomarkers. :1-17 |
| ISSN: | 1875-8592 1574-0153 |
| DOI: | 10.3233/cbm-203252 |
| Popis: | BACKGROUND: Radiotherapy is one of main useful therapies in non-small cell lung cancer (NSCLC). Nevertheless, the underlying mechanism between NSCLC cell radiosensitivity and effective treatment remains unclear. OBJECTIVE: The aim is to explore the relationship between circular (circ) RNA and NSCLC cell radiosensitivity. METHODS: CircRNA plasmacytoma variant translocation 1 (PVT1) and microRNA (miR)-1208 expression in NSCLC cells were assessed using quantitative reverse transcriptase PCR (qRT-PCR). NSCLC cells were transfected with si-PVT1 or miR-1208 inhibitor and then exposed to irradiation. Cellular biology behaviors were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL), colony formation, invasion and western blot. Additionally, binding between circPVT1 and miR-1208 was testified by dual-luciferase reporter and RIP assay. RESULTS: CircPVT1 was upregulated in NSCLC cells after irradiation treatment. Silencing circPVT1 induced inhibition of NSCLC cell growth and invasion, accompanied by cell apoptosis and γ-H2AX expression. Moreover, NSCLC cell proliferation and invasion was further inhibited by irradiation treatment in circPVT1-silenced cells, indicating a strong radiosensitivity of NSCLC cells. CircPVT1 functions as a competing endogenous RNA towards miR-1208. Silencing miR-1208 reversed NSCLC cell sensitivity response to irradiation and activated PI3K/AKT/mTOR pathway in circPVT1-silenced cells. CONCLUSIONS: Silencing circPVT1 enhanced radiosensitivity of NSCLC cells by sponging miR-1208. |
| Databáze: | OpenAIRE |
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