Up-regulation and co-expression of fibroblast growth factor receptors in human gastric cancer

Autor: Kyeong-Sun Shin, Bin-Ho Lee, Geon-Kook Lee, Jong-Ho Yang, Eun-Young Shin, Eung-Gook Kim, Sang Chul Park, Young-Jin Song, Hyo-Yung Yun
Rok vydání: 2000
Předmět:
musculoskeletal diseases
Cancer Research
medicine.medical_specialty
animal structures
Fibroblast Growth Factor 7
Angiogenesis
Biology
Fibroblast growth factor
medicine.disease_cause
chemistry.chemical_compound
Stomach Neoplasms
Internal medicine
medicine
Humans
Protein Isoforms
Receptors
Growth Factor
RNA
Messenger
Receptor
Fibroblast Growth Factor
Type 1
Receptor
Fibroblast Growth Factor
Type 2
Growth Substances
FGF10
Gene Amplification
Cancer
Receptor Protein-Tyrosine Kinases
General Medicine
medicine.disease
Blotting
Northern
Immunohistochemistry
Receptors
Fibroblast Growth Factor
Up-Regulation
Fibroblast Growth Factors
Alternative Splicing
Endocrinology
Oncology
chemistry
Fibroblast growth factor receptor
embryonic structures
Cancer research
Fibroblast Growth Factor 1
Keratinocyte growth factor
Carcinogenesis
Fibroblast Growth Factor 10
Zdroj: Journal of cancer research and clinical oncology. 126(9)
ISSN: 0171-5216
Popis: Fibroblast growth factor (FGF), a key regulatory factor of cell growth and differentiation, is involved in embryonic development, angiogenesis, and tumorigenesis. To date, four different FGF receptors (FGFRs) have been cloned and characterized. We examined the expression of four FGFRs in human gastric cancer tissues and cell lines using Northern analysis, ribonuclease protection assay, and immunohistochemistry. The mRNAs of FGFR-1 (10/14), FGFR-2 (9/14), and FGFR-4 (9/14) were up-regulated in cancer compared with normal tissues. FGFR-3 mRNAs were barely detectable in both normal and cancer tissues. These FGFR mRNAs were co-expressed in various combinations of two or three in the same tissue. Immunohistochemistry confirmed specific staining of multiple FGFRs, except FGFR-3, in the cancer specimens. To investigate the functional significance of FGFR co-expression we examined the invasive property of SNU-16 cells, which exhibited gene amplification of FGFR-2, -3, and -4 as well as over-expression of keratinocyte growth factor receptor (KGFR), a splice variant of FGFR-2, and FGFR-4 mRNA. KGF plus acidic FGF (aFGF), KGF, and aFGF treatment enhanced the invasive potential of SNU-16 cells over the control by 100%, 107%, and 47%, respectively, indicating that neither additive nor synergistic effect was induced by stimulation with aFGF plus KGF. These results suggest that co-expression of FGFRs in various combinations may cause subtle changes in the progression of gastric cancer.
Databáze: OpenAIRE
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