Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ‐1 space mission
Autor: | Changyong Wang, Jie Na, Tong Hao, Jin Zhou, Hong Li, Huimin Zhu, Peizhe Wang, Tao Zhang, Xiao-Hui Dong, Xiao-Xia Jiang, Wei-Bo Zheng, Fengzhi Zhang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Somatic cell induced pluripotent stem cells Recombinant Fusion Proteins Cell behaviour Mice Transgenic Biology 03 medical and health sciences bioreactor Mice 0302 clinical medicine Bioreactors Computer Systems Cell density Animals Cell Self Renewal Induced pluripotent stem cell Letter to the Editor Cell Proliferation Weightlessness Regeneration (biology) Cell Biology General Medicine Space Flight Embryonic stem cell space microgravity Cell biology Clone Cells 030104 developmental biology 030220 oncology & carcinogenesis regeneration Octamer Transcription Factor-3 |
Zdroj: | Cell Proliferation |
ISSN: | 1365-2184 0960-7722 |
Popis: | Induced pluripotent stem cells (iPSCs) are reprogrammed somatic cells that gained self‐renewal and differentiation capacity similar to embryonic stem cells. Taking the precious opportunity of the TianZhou‐1 spacecraft mission, we studied the effect of space microgravity (µg) on the self‐renewal capacity of iPSCs. Murine iPSCs carrying pluripotency reporter Oct4‐GFP were used. The Oct4‐EGFP‐iPSCs clones were loaded into the bioreactor and exposed to μg in outer space for 14 days. The control experiment was performed in identical device but on the ground in earth gravity (1 g). iPSCs clones were compact and highly expressed Oct4 before launch. In μg condition, cells in iPSC clones spread out more rapidly than those in ground 1 g condition during the first 3 days after launch. However, in 1 g condition, as the cell density increases, the Oct4‐GFP signal dropped significantly during the following 3 days. Interestingly, in μg condition, iPSCs originated from the spread‐out clones during the first 3 days appeared to cluster together and reform colonies that activated strong Oct4 expression. On the other hand, iPSC clones in 1 g condition were not able to recover Oct4 expression after overgrown. Our study for the first time performed real‐time imaging on the proliferation process of iPSCs in space and found that in μg condition, cell behaviour appeared to be more dynamic than on the ground. |
Databáze: | OpenAIRE |
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