Circadian variation and in vitro cytotoxic activity evaluation of volatile compounds from leaves of Piper regnellii (Miq) C. DC. var. regnellii (C. DC.) Yunck (Piperaceae)
| Autor: | Carlos R. Figueiredo, Vinicius S. Londero, Natalia Girola, Roseli R. Anderson, João Henrique G. Lago |
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| Rok vydání: | 2017 |
| Předmět: |
Stereochemistry
Plant Science Fractionation 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry HeLa Inhibitory Concentration 50 Mice Sesquiterpenes Germacrane Cell Line Tumor Circadian Clocks Oils Volatile Animals Humans Plant Oils Cytotoxic T cell Circadian rhythm IC50 Chemical composition Polycyclic Sesquiterpenes biology Traditional medicine Plant Extracts 010405 organic chemistry Chemistry Organic Chemistry Piperaceae biology.organism_classification Antineoplastic Agents Phytogenic In vitro 0104 chemical sciences Plant Leaves 010404 medicinal & biomolecular chemistry Drug Screening Assays Antitumor Piper Sesquiterpenes HeLa Cells |
| DOI: | 10.6084/m9.figshare.5277607 |
| Popis: | Aiming detection of circadian variation in the chemical composition of volatiles from Piper regnellii, the leaves were collected during four different periods (8, 12, 16 and 20 h) in the same day. After extraction by hydrodistillation and GC/MS analysis, no significant variation was observed for the main compounds: germacrene D (45.6 ± 1.5–51.4 ± 3.1%), α–chamigrene (8.9 ± 1.3–11.3 ± 2.7%) and β–caryophyllene (8.2 ± 0.9–9.5 ± 0.3%). Evaluation of in vitro cytotoxicity against several cancer and non-tumourigenic cells indicated promising activity, especially to HeLa (human cervical carcinoma) with IC50 ranging from 11 ± 3 to 17 ± 3 μg/mL. The obtained volatile oils were pooled and subjected to fractionation to afford pure β-caryophyllene, α-chamigrene and germacrene D, being this last compound the more active against HeLa cells with IC50 of 7 ± 1 μg/mL (34 ± 5 μM). Therefore, the predominance of germacrene D in all analysed oils could justify, at least in part, the activity observed for the volatile compounds from P. regnellii leaves. |
| Databáze: | OpenAIRE |
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