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The Turkish freshwater lakes, Sapanca, Iznik and Taskisi (Calticak) have been enriched with nutrients from agriculture and domestic sources for many years. A major bloom of cyanobacteria (blue-green algae) in Lake Sapanca was recorded in May 1997, closely followed by a fish kill. Investigations were subsequently made on the cyanobacteria and water quality of the lakes, including analysis for cyanobacterial hepatotoxins (microcystins) in the filtered particulate fraction. Samples, taken from the beginning of May to end of August 1998, were analysed for microcystins by high - performance liquid chromatography with photodiode array detection (HPLC-PDA), protein phosphatase inhibition assay (PPIA) and an enzyme-linked immunosorbent assay ( ELISA). No microcystins were detected in the water column in Lake Sapanca above 10 m, but toxins were found in filtered cyanobacterial samples from 20 m depth at a concentration of 3.65 mug l(-1) microcystin - LR equivalents. Ninety percent of the microcystin pool detected in L. Sapanca was found between depths of 15 and 25 m. The principal microcystin detected by HPLC-PDA was similar to microcystin - RR. Two unidentified microcystin variants were found in Lake Taskisi surface samples at a concentration of 2.43 mug l(-1) microcystin - LR equivalents in the filtered cyanobacterial cell fraction. Although 10 water samples ( 10 x 5 l) were taken from Lake Iznik ( surface to 20 m, 5 m intervals), no microcystins were detected by HPLC-PDA ( limit of detection 10 ng). The depth at which microcystins were detected in L. Sapanca coincided with the draw-off depth for the drinking water supply for the city of Sakarya. |
