Reduced graphene-oxide transducers for biosensing applications beyond the Debye-screening limit
| Autor: | Sven Ingebrandt, Xianping Chen, Pawan Jolly, Xiaoling Lu, Vivek Pachauri, Anna Miodek, Walid-Madhat Munief, Pedro Estrela |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Aptamer Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences In-line electrochemical impedance spectroscopy law.invention Limit of Detection law Electrochemistry Humans ddc:610 Thin film chemistry.chemical_classification Reduced graphene-oxide thin films Graphene business.industry Biomolecule 010401 analytical chemistry Aptasensor Electrochemical Techniques General Medicine Aptamers Nucleotide Prostate-Specific Antigen 021001 nanoscience & nanotechnology 0104 chemical sciences Dielectric spectroscopy Transducer Semiconductor chemistry Graphite Debye-screening limitation 0210 nano-technology business Biosensor Biotechnology |
| Zdroj: | Lu, X, Miodek, A, Munief, W, Jolly, P, Pachauri, V, Chen, X, Estrela, P & Ingebrandt, S 2019, ' Reduced Graphene-Oxide Transducers for Biosensing Applications Beyond the Debye-Screening Limit ', Biosensors and Bioelectronics, vol. 130, pp. 352-359 . https://doi.org/10.1016/j.bios.2018.09.045 Biosensors and bioelectronics 130, 352-359 (2019). doi:10.1016/j.bios.2018.09.045 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.09.045 |
| Popis: | In the field of label-free biosensing, various transducer materials and strategies are under investigation to overcome the Debye-screening limitation of charged biomolecules. We demonstrate an in-line, impedimetric aptasensor with reduced graphene-oxide (rGO) thin films as transducers to detect prostate specific antigens (PSA) in a physiological buffer solution. Unlike classical electrochemical impedance spectroscopy (EIS), this direct, label-free and fully-electronic biosensor approach does not need any redox markers. As specific capture molecules, short anti-PSA aptamers ensured a close binding of the target molecules to the transducer surfaces. Results showed a limit of detection smaller than 33 pM of PSA and a wide detection range from 0.033 to 330 nM fully covering the clinically relevant range of PSA (0.115–0.290 nM). This promising performance can be attributed to the bipolar electronic transport characteristics of the ultra-thin rGO layers similar to pristine graphene. The attachment of target biomolecules to the films changes the resistance of the rGO thin films. Such an in-line EIS configuration with rGO thin films opens promising prospects for biosensing beyond the Debye-screening limitation, which is a major challenge for conventional semiconductor field-effect devices towards clinical applications. |
| Databáze: | OpenAIRE |
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