Histological validation of DW-MRI tractography in human postmortem tissue
Autor: | Ralf A. W. Galuske, Rainer Goebel, Oriana Chiry, Hansjürgen Bratzke, Dae-Shik Kim, Itamar Ronen, Alard Roebroeck, Arne Seehaus |
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Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), Cognitive Neuroscience, RS: FPN CN 1 |
Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Cognitive Neuroscience
Sensitivity and Specificity 030218 nuclear medicine & medical imaging 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neural Pathways Fractional anisotropy Image Processing Computer-Assisted medicine Humans Diffusion Tractography human brain single pathways Dti tractography fluorescent dyes medicine.diagnostic_test Receiver operating characteristic business.industry Brain Magnetic resonance imaging Articles diffusion tensor imaging receiver operating characteristics Diffusion Magnetic Resonance Imaging ROC Curve Area Under Curve Postmortem tissue Autopsy Psychology Nuclear medicine business 030217 neurology & neurosurgery Biomedical engineering Diffusion MRI Tractography |
Zdroj: | Cerebral Cortex, 23, 442-450. Oxford University Press Cerebral Cortex, 23(2), 442-450 Cerebral Cortex Cerebral Cortex, 23(2), 442-450. Oxford University Press |
ISSN: | 1047-3211 |
Popis: | Despite several previous attempts, histological validation of diffusion-weighted magnetic resonance imaging (DW-MRI)-based tractography as true axonal fiber pathways remains difficult. In the present study, we establish a method to compare histological and tractography data precisely enough for statements on the level of single tractography pathways. To this end, we used carbocyanine dyes to trace connections in human postmortem tissue and aligned them to high-resolution DW-MRI of the same tissue processed within the diffusion tensor imaging (DTI) formalism. We provide robust definitions of sensitivity (true positives) and specificity (true negatives) for DTI tractography and characterize tractography paths in terms of receiver operating characteristics. With sensitivity and specificity rates of approximately 80%, we could show a clear correspondence between histological and inferred tracts. Furthermore, we investigated the effect of fractional anisotropy (FA) thresholds for the tractography and identified FA values between 0.02 and 0.08 as optimal in our study. Last, we validated the course of entire tractography curves to move beyond correctness determination based on pairs of single points on a tract. Thus, histological techniques, in conjunction with alignment and processing tools, may serve as an important validation method of DW-MRI on the level of inferred tractography projections between brain areas. |
Databáze: | OpenAIRE |
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