Differential expression of phospholipase C specific for inositol phospholipids at the cell surface of rat glial cells and REF52 rat embryo fibroblasts
| Autor: | J J Volwerk, O. H. Griffith, K K Hedberg, Birrell Gb |
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| Rok vydání: | 1993 |
| Předmět: |
Phospholipase
Biology Phosphatidylinositols Biochemistry Cell Line Substrate Specificity Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Tumor Cells Cultured Animals Insulin Phosphatidylinositol Inositol phosphate Cells Cultured chemistry.chemical_classification Cerebral Cortex Phospholipase C Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase fungi Cell Membrane Glioma Fibroblasts Embryo Mammalian Molecular biology Rats Kinetics medicine.anatomical_structure chemistry Cell culture Astrocytes Neuroglia Intracellular Cell Division Astrocyte |
| Zdroj: | Journal of neurochemistry. 60(2) |
| ISSN: | 0022-3042 |
| Popis: | Phosphatidylinositol(PI)-specific phospholipase C activity was detected on the surface of rat astrocytes, rat C6 glioma cells, and rat embryo (REF52) fibroblasts. The cell surface phospholipase C (ecto-PLC) activity was calcium-dependent, did not result from secreted phospholipase C, and was not released from the cell surface by bacterial PI-specific phospholipase C. Agents known to stimulate intracellular PI turnover, including carbachol, L-glutamic acid, acetylcholine, and orthovanadate, did not induce measurable alterations in the activity of the ecto-PLC. The expression of ecto-PLC activity by REF52 fibroblasts was density-dependent: subconfluent cultures of REF52 exhibited low levels of activity (less than 80 pmol of inositol phosphate formed/min/10(6) cells), whereas in confluent cultures ecto-PLC activity increased to approximately 300 pmol/min/10(6) cells. In contrast to this behavior and that exhibited by previously reported ecto-PLC-positive cell types, the ecto-PLC activity exhibited by astrocytes (approximately 1,000 pmol/min/10(6) cells) and by C6 glioma cells (approximately 100 pmol/min/10(6) cells) was independent of cell culture density up to confluence. The constitutive expression of ecto-PLC activity of astroglial cells may be related to their function as accessory cells in close association with neurons. |
| Databáze: | OpenAIRE |
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