A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides
| Autor: | Anastasia S. Sheremet, Sergey V. Gronskiy, Natalia Pavlovna Zakataeva, Anna E. Novikova, Vitaliy Arkadyevich Livshits, Ekaterina Aleksandrovna Kutukova |
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| Rok vydání: | 2007 |
| Předmět: |
Purine
Guanosine Purine nucleoside phosphorylase Bacillus subtilis Biology Microbiology chemistry.chemical_compound Bacterial Proteins Escherichia coli medicine Cloning Molecular Inosine Purine metabolism Molecular Biology Hypoxanthine Purine Nucleosides General Medicine biology.organism_classification Molecular biology Purine-Nucleoside Phosphorylase chemistry Biochemistry Purines Heterologous expression medicine.drug |
| Zdroj: | Research in Microbiology. 158:659-665 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2007.08.003 |
| Popis: | The pbuE (ydhL) gene from Bacillus subtilis is known to encode the purine base efflux pump, and its expression is controlled by an adenine-dependent riboswitch. We cloned the pbuE gene from Bacillus amyloliquefaciens and examined gene expression by its own cis-acting regulatory elements in Escherichia coli. Regulation of pbuE expression, previously found in B. subtilis, was retained in this heterologous expression: it was induced by adenine and activated by a mutation in the 5' untranslated region, which disrupted transcription termination. This observation supports the model that the adenine-dependent riboswitch directly regulates pbuE expression, without requiring additional factors. Overexpression of the PbuE pump conferred upon the E. coli strain resistance to higher concentrations of inosine, adenosine and guanosine, and increased exogenous inosine accumulation by E. coli cells deficient in purine nucleoside phosphorylase. Overexpression of the PbuE pump also enhanced hypoxanthine excretion by the E. coli hypoxanthine-producing strain and inosine excretion both by the E. coli and B. amyloliquefaciens nucleoside-producing strains. Thus, for the first time, we obtained direct evidence for the involvement of PbuE in efflux of not only purine bases, but also purine ribonucleosides. A possible new role for the pump in cell physiology is discussed. |
| Databáze: | OpenAIRE |
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