Clinical Evaluation of the BD FACSPresto™ Near-Patient CD4 Counter in Kenya
| Autor: | Henok Tilahun, Imelda Omana-Zapata, Beverly Lu, Benta Akoth, Paul Omolo, Scott Bornheimer, Francis Angira, Valarie Opollo, Kevin A. Judge, Clement Zeh |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male RNA viruses Multiple days Physiology lcsh:Medicine HIV Infections Pathology and Laboratory Medicine White Blood Cells 0302 clinical medicine Immunodeficiency Viruses Animal Cells Medicine and Health Sciences 030212 general & internal medicine Lymphocytes lcsh:Science Child Multidisciplinary T Cells Anemia Venous blood Hematology Middle Aged Flow Cytometry Body Fluids Blood Medical Microbiology Child Preschool Viral Pathogens Viruses Female Lymph Anatomy Cellular Types Pathogens Clinical evaluation Research Article Adult Adolescent Coefficient of variation Immune Cells Immunology Sensitivity and Specificity Microbiology 03 medical and health sciences Deming regression Young Adult Retroviruses medicine Humans Microbial Pathogens Aged Blood Cells business.industry lcsh:R Lentivirus Organisms Biology and Life Sciences HIV Cell Biology medicine.disease 030112 virology Kenya CD4 Lymphocyte Count Capillaries Blood Counts Cardiovascular Anatomy Blood Vessels lcsh:Q Hemoglobin business Nuclear medicine |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 8, p e0157939 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Background The BD FACSPresto™ Near-Patient CD4 Counter was developed to expand HIV/AIDS management in resource-limited settings. It measures absolute CD4 counts (AbsCD4), percent CD4 (%CD4), and hemoglobin (Hb) from a single drop of capillary or venous blood in approximately 23 minutes, with throughput of 10 samples per hour. We assessed the performance of the BD FACSPresto system, evaluating accuracy, stability, linearity, precision, and reference intervals using capillary and venous blood at KEMRI/CDC HIV-research laboratory, Kisumu, Kenya, and precision and linearity at BD Biosciences, California, USA. Methods For accuracy, venous samples were tested using the BD FACSCalibur™ instrument with BD Tritest™ CD3/CD4/CD45 reagent, BD Trucount™ tubes, and BD Multiset™ software for AbsCD4 and %CD4, and the Sysmex™ KX-21N for Hb. Stability studies evaluated duration of staining (18–120-minute incubation), and effects of venous blood storage |
| Databáze: | OpenAIRE |
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