Troponin Aptamer on an Atomically Flat Au Nanoplate Platform for Detection of Cardiac Troponin I
| Autor: | Hyunsoo Lee, Hyoban Lee, Youngdong Yoo, Weon Kim, Jeong Young Park, Hyungjun Youn, Taejoon Kang, Bongsoo Kim, Ahreum Hwang, Changill Ban |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
surface-enhanced Raman scattering
Cardiac troponin General Chemical Engineering Aptamer 02 engineering and technology 010402 general chemistry 01 natural sciences Article lcsh:Chemistry Present method Troponin I nanoplate General Materials Science cardiovascular diseases biology Chemistry Atomic force microscopy aptamer 021001 nanoscience & nanotechnology Troponin 0104 chemical sciences lcsh:QD1-999 cTnI biology.protein Biophysics Biomarker (medicine) 0210 nano-technology Biosensor |
| Zdroj: | Nanomaterials Volume 10 Issue 7 Nanomaterials, Vol 10, Iss 1402, p 1402 (2020) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10071402 |
| Popis: | Well-ordered bioreceptors on atomically flat Au surfaces can be a high-performance biosensor. Cardiac troponin I proteins (cTnIs) have been regarded as a specific biomarker for acute myocardial infarction (AMI). Here, we report the accurate detection of cTnIs using an aptamer-immobilized Au nanoplate platform. The single-crystalline and atomically flat Au nanoplate was characterized by atomic force microscopy. For the precise detection of cTnI, we immobilized an aptamer that can strongly bind to cTnI onto an atomically flat Au nanoplate. Using the aptamer-immobilized Au nanoplate, cTnIs were successfully detected at a concentration of 100 aM (2.4 fg/mL) in buffer solution. Furthermore, cTnIs in serum could be identified at a concentration of 100 fM (2.4 pg/mL). The total assay time was ~7 h. Importantly, the aptamer-immobilized Au nanoplate enabled us to diagnose AMI patients accurately, suggesting the potential of the present method in the diagnosis of AMI. |
| Databáze: | OpenAIRE |
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