Structural Organization and Chromosomal Localization of a Human Gene (HIP/PAP) Encoding a C-type Lectin Overexpressed in Primary Liver Cancer

Autor: Philippe Vernier, Chantal Lasserre, M. Simon, Van Cong Nguyen, Christian Bréchot, Hitoshi Ishikawa, Laurence Christa, Sylvie Diriong
Přispěvatelé: PERIGNON, Alain, INSERM U90 [CHU Necker-Enfants Malades, AP-HP], Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de Cytogénetique et Génétique Oncologiques [Villejuif], Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Institut Alfred Fessard, Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 1994
Předmět:
MESH: Sequence Analysis
DNA
Transcription
Genetic
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Restriction Mapping
[SDV.NEU.PC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Antigens
Neoplasm
Pancreatitis-Associated Proteins
MESH: Amino Acid Sequence
Regulatory Sequences
Nucleic Acid
MESH: Base Sequence
Biochemistry
MESH: Nucleic Acid Hybridization
Exon
Mice
MESH: Lectins
C-type lectin
MESH: Liver Neoplasms
Lectins
Gene duplication
MESH: Blotting
Southern
MESH: Animals
MESH: Proteins
Cloning
Molecular
MESH: Phylogeny
Phylogeny
MESH: Restriction Mapping
Genomic organization
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
Liver Neoplasms
Chromosome Mapping
Nucleic Acid Hybridization
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Blotting
Southern
medicine.anatomical_structure
Chromosomes
Human
Pair 2
MESH: Protein Biosynthesis
MESH: Biomarkers
Tumor
Pancreas
Signal peptide
Molecular Sequence Data
MESH: Chromosomes
Human
Pair 2
Biology
Cell Line
Antigens
Neoplasm
medicine
Biomarkers
Tumor
Animals
Humans
MESH: Regulatory Sequences
Nucleic Acid
Lectins
C-Type
MESH: Cloning
Molecular
Amino Acid Sequence
RNA
Messenger
MESH: Pancreatitis-Associated Proteins
Gene
MESH: Mice
MESH: RNA
Messenger
MESH: Humans
MESH: Molecular Sequence Data
Base Sequence
MESH: Transcription
Genetic
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Chromosome
Proteins
Sequence Analysis
DNA
Molecular biology
MESH: Cell Line
Protein Biosynthesis
Immunology
MESH: Chromosome Mapping
[SDV.NEU.SC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
MESH: Lectins
C-Type
Zdroj: European Journal of Biochemistry
European Journal of Biochemistry, 1994, 224 (1), pp.29-38. ⟨10.1111/j.1432-1033.1994.tb19991.x⟩
ISSN: 0014-2956
DOI: 10.1111/j.1432-1033.1994.tb19991.x⟩
Popis: International audience; We previously identified, through differential screening of a human primary liver cancer library, a novel gene (named HIP) the expression of which is markedly increased in 25% of human primary liver cancers. HIP mRNA expression is tissue specific since it is restricted to pancreas and small intestine. HIP protein consists in a signal peptide linked to a carbohydrate-recognition domain (CRD), typical of C-type lectins without other binding domains. We have proposed that HIP and related proteins belong to a new family of C-type lectins. Drickamer [Drickamer, K. (1993) Curr. Opin. Struct. Biol. 3,393-400] included this group of proteins in his classification of C-type lectins as the free CRD (group VII) lectins. In the present report we describe the genomic organization and the chromosomal localization of HIP. We have shown that HIP is in fact the pancreatitis-associated protein (PAP) and provided a phylogenetic analysis of the free CRD lectins. Furthermore, the analysis of HIP/PAP gene indicates that the HIP/PAP CRD is encoded by four exons, a pattern shared with all members of this group of proteins. This common intron-exon organization indicates an ancient divergence of the free CRD-lectin group from other groups of C-type lectins. We provide evidence for the localization of HIP/PAP on chromosome 2, suggesting previous duplication of HIP/PAP and the related reg I alpha and reg I beta genes from the same ancestral gene. Finally, the sequence of the 5' upstream region of the HIP gene shows several potential regulatory elements which might account for the enhanced expression of the gene during pancreatic inflammation and liver carcinogenesis.
Databáze: OpenAIRE
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