Pt(II)-Thiocarbohydrazone Complex as Cytotoxic Agent and Apoptosis Inducer in Caov-3 and HT-29 Cells through the P53 and Caspase-8 Pathways
| Autor: | Ghassan M. Sulaiman, Mohanad Mousa Kareem, Abeer A. AlObaid, Abeer A. Ibrahim, Zainab J. Taqi, Taghreed H. Al-Noor, Tahani I. Al-Muhimeed, Salim Albukhaty, Majid S. Jabir, Usama I. Sahib |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
p53 Cell cycle checkpoint Pharmaceutical Science DNA fragmentation 02 engineering and technology Caspase 8 Article caspase-8 03 medical and health sciences Pharmacy and materia medica Drug Discovery Cytotoxic T cell Cytotoxicity Chemistry apoptosis Cell cycle 021001 nanoscience & nanotechnology Molecular biology RS1-441 030104 developmental biology Pt(II) complex anticancer activity Apoptosis Cancer cell Medicine Molecular Medicine 0210 nano-technology |
| Zdroj: | Pharmaceuticals Volume 14 Issue 6 Pharmaceuticals, Vol 14, Iss 509, p 509 (2021) |
| ISSN: | 1424-8247 |
| DOI: | 10.3390/ph14060509 |
| Popis: | In this study, a platinum(II) complex ([Pt(H2L)(PPh3)] complex) containing a thiocarbohydrazone as the ligand was tested as an anti-proliferative agent against ovarian adenocarcinoma (Caov-3) and human colorectal adenocarcinoma (HT-29) through MTT assays. Apoptotic markers were tested by the AO/PI double staining assay and DNA fragmentation test. Flow cytometry was conducted to measure cell cycle distribution, while the p53 and caspase-8 pathways were tested via immunofluorescence assay. Results demonstrated that the cytotoxic effect of the Pt(II)-thiocarbohydrazone complexes against Caov-3 and HT-29 cells was highly significant, and this effect triggered the activation of the p53 and caspase-8 pathways. Besides, apoptosis stimulated by the Pt(II)-thiocarbohydrazone complex was associated with cell cycle arrest at the G0/G1 phase. These findings suggest that the target complex inhibited the proliferation of Caov-3 and HT-29 cells, resulting in the arrest of the cell cycle and induction of apoptosis via the stimulation of the p53 and caspase-8 pathways. The present data suggests that the Pt(II)-thiocarbohydrazone complex could also be a promising chemotherapeutic agent for other types of cancer cells. |
| Databáze: | OpenAIRE |
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