Biosynthesis of Taxol: Enzymatic Acetylation of 10-Deacetylbaccatin-III to Baccatin-III in Crude Extracts from Roots ofTaxus baccata
| Autor: | Bettina Kammer, Christine Hacker, Till Hornbogen, Wolfram Weckwerth, Dietrich Ewald, Rainer Zocher |
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| Rok vydání: | 1996 |
| Předmět: |
10-Deacetylbaccatin
Paclitaxel Stereochemistry Biophysics Plant Roots Biochemistry High-performance liquid chromatography chemistry.chemical_compound Alkaloids Biosynthesis Cambium Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Plants Medicinal Cell-Free System biology Acetylation Cell Biology biology.organism_classification Antineoplastic Agents Phytogenic Triterpenes Enzyme Taxus chemistry Baccatin III Taxoids |
| Zdroj: | Biochemical and Biophysical Research Communications. 229:16-20 |
| ISSN: | 0006-291X |
| Popis: | The biosynthesis of taxol is a multistep process. One intermediate reaction is the acetylation of 10-deacetylbaccatin-III (10-DAB) to baccatin-III, an assumed precursor of taxol. Here we describe the cell free acetylation of 10-DAB in crude extracts from roots ofTaxus baccatasaplings using14C- or3H-labeled acetyl-coenzyme A as the acetyl donor. The reaction is strictly dependent on the addition of 10-DAB and is specific for the 10-hydroxyl group of the taxane ring. Formation of radiolabeled baccatin-III was confirmed by co-chromatography of the labeled product with authentic baccatin-III in different TLC-systems and HPLC. Furthermore, the acetylation product showed an identical UV spectrum as authentic baccatin-III. Crude extracts from cambium of stems yielded three- to fivefold lower activity. This is in agreement with our finding that the taxol titer in roots was considerably higher than that in cambium. |
| Databáze: | OpenAIRE |
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