Scanning electron microscopy in the identification of fly artifacts
| Autor: | Desiree Martini, Alberto Amadasi, Laura Ingrà, Mirella Falconi, Susi Pelotti, Maria Carla Mazzotti, Guido Pelletti, Paolo Fais, Chiara Palazzo |
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| Přispěvatelé: | Pelletti G., Mazzotti M.C., Fais P., Martini D., Ingra L., Amadasi A., Palazzo C., Falconi M., Pelotti S. |
| Rok vydání: | 2018 |
| Předmět: |
Scanning electron microscope
Sarcophagidae Sarcophaga carnaria 01 natural sciences Pathology and Forensic Medicine Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Animals Humans 030216 legal & forensic medicine biology Chemistry 010401 analytical chemistry Bloodstain pattern analysi Sem analysis biology.organism_classification Blood Physiological Phenomena 0104 chemical sciences Fly artifact Blood Stains Microscopy Electron Scanning Artifacts Bloodstain pattern analysis Scanning electron microscopy Biomedical engineering Crime scene |
| Zdroj: | International journal of legal medicine. 133(5) |
| ISSN: | 1437-1596 |
| Popis: | Bloodstain pattern analysis has a key role in crime scene reconstruction; however, it can be hampered by diverse confounding factors, such as insect activity which may lead to the production of small artifactual bloodstains, commonly referred to as fly artifacts (FA). Although several techniques aimed at distinguishing human bloodstains and FA have been developed, actually, no standardized and reproducible methodology is available. The aim of our study was to test the use of scanning electron microscopy (SEM) to distinguish human bloodstains from FA produced by Sarcophaga carnaria. FA and bloodstains have been produced on five different deposition surfaces under experimental conditions. After visual analysis, bloodstains and FA were analyzed at standard low (× 40–× 300) and high (× 600–× 1200) magnification through a Philips SEM 515. Although differential diagnosis between bloodstains and FA resulted often inconclusive at visual analysis, SEM analysis allowed the identification of additional key distinctive morphological features. In particular, on the surface of FA, small crystal-like and/or amorphous material deposits were observed. Such deposits were absent on bloodstains which, on the other hand, displayed red blood cells stacked in “rouleaux.” Basing on these results and under our experimental conditions, SEM analysis resulted suitable to perform a differential diagnosis between bloodstains and FA produced from the insect activity of Sarcophaga carnaria. |
| Databáze: | OpenAIRE |
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