Effects of Ozone on Injury after Gamma Knife Radiosurgery
| Autor: | Zeynep Cingu Meric, Ibrahim M. Ziyal, Ayca Arslanhan, Semra Unal, Süheyla Uyar Bozkurt, Selin Tural Emon |
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| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
Pathology medicine.medical_specialty Necrosis Vascular permeability Vasodilation Brain Edema Radiosurgery Capillary Permeability 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ozone Edema Parenchyma Medicine Animals business.industry Brain Endothelial Cells Rats Vascular endothelial growth factor chemistry Vacuolization Blood-Brain Barrier 030220 oncology & carcinogenesis Basigin Immunohistochemistry Surgery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | World neurosurgery. 149 |
| ISSN: | 1878-8769 |
| Popis: | Background At present, gamma knife radiosurgery plays an important role in neurosurgical procedures. Gamma knife radiosurgery has been used to treat many types of brain tumors and as a functional intervention. However, gamma knife treatment has a devastating effect on the normal brain parenchyma surrounding the target point. It causes increased vascular permeability, vasodilation, and swelling in endothelial cells. Ozone has antioxidant, antiapoptotic, and anti-inflammatory effects in the body. Thus, we evaluated the radioprotective effects of ozone in rats undergoing gamma knife radiation. Methods In the present study, 24 Sprague-Dawley male rats weighing 250–300 g in 3 groups of 8 rats each were used. The rats were selected randomly. The control group did not receive any gamma knife radiation. The other 2 groups received 50 Gy of radiation, with 1 group given ozone treatment and the other group not given ozone treatment after gamma knife radiosurgery. At 12 weeks after gamma knife radiation, the rats were sacrificed with high-dose anesthetic agents and the tissues prepared for evaluation. The slides were evaluated for necrosis, vacuolization, glial proliferation, and vascular proliferation using hematoxylin-eosin staining. Vascular endothelial growth factor (VEGF) and extracellular matrix metalloproteinase inducer (also known as CD147) were evaluated using immunohistochemical staining. Results VEGF expression in glial tissue was significantly less in the group receiving ozone (χ2 = 15.00; df = 4; P = 0.005) compared with the group that had not received ozone and was similar to the expression in the control group. Conclusions The lower expression of VEGF in the group receiving ozone might cause less edema in the surrounding tissue owing to less degradation of vascular permeability in the rat brain tissue. |
| Databáze: | OpenAIRE |
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