PTENP1 acts as a ceRNA to regulate PTEN by sponging miR-19b and explores the biological role of PTENP1 in breast cancer
| Autor: | Luo Wh, Gao J, Li Rk, Guo Lh, Huang Gq |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Apoptosis Breast Neoplasms 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Genes Reporter Annexin RNA interference Cell Line Tumor Humans PTEN 3' Untranslated Regions Molecular Biology Cell Proliferation Regulation of gene expression biology Chemistry Competing endogenous RNA Cell growth PTEN Phosphohydrolase Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Molecular Medicine Female RNA Interference RNA Long Noncoding |
| Zdroj: | Cancer Gene Therapy. 24:309-315 |
| ISSN: | 1476-5500 0929-1903 |
| DOI: | 10.1038/cgt.2017.29 |
| Popis: | This study aimed to investigate role of long noncoding RNA PTENP1 regulating PTEN expression via miR-19b to affect breast cancer (BC) progression. We measured expressions of PTENP1, miR-19b and PTEN in 65 matched BC cancerous and noncancerous tissues by quantitative real-time fluorescence PCR (qRT-PCR) and investigated the biological effects of PTENP1 in BC MDA-MB-231 cells by several in vitro experiments including CCK8, wound healing, transwell and Annexin V-FITC/PI analysis. Besides, the competing endogenous RNA (ceRNA) activity of PTENP1 on miR-19b was detected by luciferase reporter assay, and the expressions of related genes and proteins were determined by western blot assay and qRT-PCR. Increased PTENP1 and PTEN and decreased miR-19b were observed in BC tissues and cell lines. Further, PTENP1 and PTEN are direct targets of miR-19b, and overexpressed PTENP1 in MDA-MB-231 cells could supress cell proliferation, migration and invasion and promote cell apoptosis. Moreover, PTENP1 could upregulate PTEN via its ceRNA interaction on miR-19b, as well as induced the upregulation of p53 and downregulation of p-AKT. Enhanced PTENP1 could inhibit BC cell growth, metastasis and tumourigenicity by inhibiting miR-19b and facilitating PTEN in BC, thereby may represent a novel target for diagnosis and treatment of BC. |
| Databáze: | OpenAIRE |
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