Pesticide induced alterations in gene expression in the lobster, Homarus americanus

Autor: Anna N. Walker, Patrick Larkin, Ernest S. Chang, Parshall B. Bush, Michael N. Horst, Thomas G. Wilson, Tim Miller
Rok vydání: 2007
Předmět:
Zdroj: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 2:44-52
ISSN: 1744-117X
DOI: 10.1016/j.cbd.2006.11.004
Popis: Using subtractive hybridization, we have identified 17 genes that are either up- or down-regulated in the hepatopancreas (Hp) of the lobster, Homarus americanus , by acute exposure to the juvenile hormone analog methoprene. The expression of some of the genes obtained from the subtraction libraries was confirmed by real time Q-PCR experiments. These genes encode several different classes of proteins including: structural, enzymatic and regulatory polypeptides. Enzymes represent the predominant genes up-regulated by methoprene. Included in this group are betaine-homocysteine S -methyltransferase (BHMT) and two other enzymes of the methionine cycle. Increased expression of a translation factor (eIF2), as well as of cytosolic (aldose reductase), structural (β-tubulin, L5A) and plasma membrane (CD42d) proteins was observed. In addition, a major feature of altered gene expression in methoprene treated Hp was increased levels of enzymes associated with protein turnover, including trypsin, ubiquitin conjugating enzyme and ubiquitin carboxyl terminal hydrolase. Down-regulation of the members of the hemocyanin family was observed. Assays confirmed elevated levels of trypsin in the Hp of lobsters after 24 h exposure to methoprene. Our findings suggest a wide variety of cellular targets are altered by methoprene.
Databáze: OpenAIRE
Externí odkaz: