Application of coherent anti-stokes Raman scattering microscopy to image the changes in a paclitaxel-poly(styrene-b-isobutylene-b-styrene) matrix pre- and post-drug elution
| Autor: | James J. Barry, Eunah Kang, Kalpana R. Kamath, Kinam Park, Ji-Xin Cheng, Haifeng Wang, Il Keun Kwon, Young-Ho Song, Kathleen M. Miller |
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| Rok vydání: | 2008 |
| Předmět: |
Chemical imaging
Materials science Paclitaxel Polymers Surface Properties Scanning electron microscope Biomedical Engineering Analytical chemistry Biocompatible Materials Spectrum Analysis Raman Styrenes Styrene Biomaterials chemistry.chemical_compound symbols.namesake Drug Delivery Systems Materials Testing Microscopy chemistry.chemical_classification Drug Carriers Elution Metals and Alloys Polymer Antineoplastic Agents Phytogenic chemistry Ceramics and Composites symbols Stents Raman scattering |
| Zdroj: | Journal of Biomedical Materials Research Part A. :913-920 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.31813 |
| Popis: | Mapping the drug distribution in a polymeric film and following the subsequent changes that result during and after drug release is important to better understand the mechanism of drug release. This under- standing leads to more efficiently designed tailor-made release profiles for drug-containing biomedical devices. Coherent anti-Stokes Raman scattering (CARS) micros- copy was used for in situ imaging of local drug distribu- tion in polymeric films, taking advantage of the three- dimensional (3D) resolution, high speed, high sensitivity, and noninvasiveness of the technology. Additionally, the morphological changes of poly(styrene-b-isobutylene-b- styrene) (SIBS) films during paclitaxel release were char- acterized by scanning electron microscopy, and drug release was quantitatively determined by high perform- ance liquid chromatography. The time-dependent changes in the 3D distribution of paclitaxel in the polymer film were visualized using CARS microscopy. CARS images showed that the paclitaxel was uniformly distributed throughout the SIBS matrix. Changes in the paclitaxel dis- tribution during release were monitored using depth in- tensity profiles and showed that, upon exposure of the paclitaxel-loaded film to a release medium, the quantita- tive CARS intensity of paclitaxel decreased. These results indicate that paclitaxel was dissolved and depleted from the SIBS film during in vitro drug elution, supporting the use of CARS microscopy as an effective nondestructive technique for chemical imaging of paclitaxel elution dy- namics in polymer films. 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 913-920, 2008 |
| Databáze: | OpenAIRE |
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