Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation
| Autor: | Mehrzad Mesbah, Taravat Molayemraftar, Takavar Mohammadian, Abdolhossein Jangaran Nejad, Reza Ghanei-Motlagh, Hamzeh Mohtashamipour, Mojtaba Zarea |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment Cyprinidae Gene Expression Aquatic Science Biology Proinflammatory cytokine Random Allocation 03 medical and health sciences Basal (phylogenetics) Immune system Internal medicine Gene expression medicine Animals Environmental Chemistry Mannan chemistry.chemical_classification Innate immune system Dose-Response Relationship Drug Prebiotic 04 agricultural and veterinary sciences General Medicine Oligosaccharide Animal Feed Immunity Innate Diet Gastrointestinal Microbiome Prebiotics 030104 developmental biology Endocrinology chemistry Dietary Supplements 040102 fisheries Cytokines 0401 agriculture forestry and fisheries |
| Zdroj: | Fish & Shellfish Immunology. 112:38-45 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2021.02.012 |
| Popis: | In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus). Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days. According to the results, growth parameters were significantly improved in fish fed with prebiotic (1 and 1.5%) for 90 days (p 0.05). The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p 0.05). Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp. in the prebiotic-treated groups relative to the control group (p 0.05). Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60. Serum bactericidal and lysozyme activities were significantly (p 0.05) elevated after feeding with dietary prebiotic at all intervals (days 30, 60 and 90). However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p 0.05). The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals. The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout. |
| Databáze: | OpenAIRE |
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