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The effect of Saccharum barberi on total serum protein and serum albumin in albino wistar rats and Guinea pigs were determined. An ethanolic extract of saccharum barberi was made. Twenty (20) Albino wistar rats and twenty Guinea pigs of both male and female were used for the experiment. The albino wistar rats and Guinea pigs were divided into four groups (A – D), each group made up of five (5) animals Group A is the control while B, C, D were the test animals. The test animal were given a dose level of 100mg/kg, 200mg/kg and 300mg/kg body weight respectively from the prepared crude ethanolic extract of saccharum barberi for a period of twenty one (21) days. The blood sample of each animal (Albino wistar rats and Guinea pigs) were taken and the total serum protein determined by Biuret method while the serum albumin was determined using the Bromocresol green method. The results shows that the saccharum barberi has the effect of lowering the total serum protein and the serum albumin, although more pronounced in the albino wistar rats than the Guinea pigs. It is equally dose dependent. The saccharum barberi could be used in treating ailments that is caused by excess protein. Keywords: Saccharum barberi, albino rats, bromocresol, biuret method, wister rats and guinea pigs REFERENCES Begun, SN (2002). Terpenoids from the stem of saccharum barberi phytochemistry 61(4):399-400. Doumas, BJ and Bigas, HG (2002). Standard method of evaluation of clinical chemistry N.Y 7:175. Doumas, BJ and Biggs HG (2002). Standard method of evaluation of clinical chemistry N.Y 7:175. Duke, AL (1978). Anti-diarrhea evaluation of some medicinal plants used by Zulu traditional healers. Journal of Ethanopharmacology 99(1): 53-56. Gibbon, GF (2002). Lipoproteins and membrane biochemistry of lipids, 4th ed., 573-597. Swapna Gurrapu and Estari Mamidala. Medicinal Plants Used By Traditional Medicine Practitioners in the Management of HIV/AIDS-Related Diseases in Tribal Areas of Adilabad District, Telangana Region. The Ame J Sci & Med Res. 2016:2(1):239-245. doi:10.17812/ajsmr2101. Gornal, AU, Bardewiu, LJ and David, MA (1949). Determination of serum protein by mean of Biuret reaction J. Biological Chemistry. 177:751. Harper, FC and Dugaiezyk, E (2003). Protein measurement with phenol reagent: Journal of biological chemistry 193-265. Hawkins, V and Dugaiezyk, E (2002). The targeting and assembly of peroribosomals proteins. Trend Biochem. Sci. 21:54. Hayashi, T, Okamika K, Kawasaki, M and Monta, N (1993). Production of Ditterpenoids by cultured cells from two chemotypes scoparla dulcis phytochemistry 35(2): Marcphason, IG and Evarald, DW (1972). Serum albumin estimation. Modification of Bromocresol green method. Journal of clinical chemistry Act 37:117-121. Smith, R, South Wellkeely, J and Chester, D (2005). Should serum pancreatic lipase replace serum amylase as a biomarker of acute pancreatic ANZ J. Surg 75(6): 399-404. |
