Effects of granulocyte colony-stimulating factor and interferon-γ on antifungal activity of human polymorphonuclear neutrophils against pseudohyphae of different medically important Candida species

Autor: Andrew Holmes, Emmanuel Roilides, Thomas J. Walsh, Cassann Blake, Philip A. Pizzo
Rok vydání: 1995
Předmět:
Zdroj: Journal of Leukocyte Biology. 57:651-656
ISSN: 1938-3673
0741-5400
DOI: 10.1002/jlb.57.4.651
Popis: Polymorphonuclear neutrophils (PMNs) are the major host defense against pseudohyphae, the inva sive form of Candida species. We studied the effects of granulocyte colony-stimulating factor (G-CSF) and interfcron-γ (IFN-γ) on the PMN-induced damage of pseudohyphae of Candida albicans, Candida tropicalis, and Candida parapsilosis in vitro by using two antifungal assays: a modified limiting dilution assay and a colorimet ric metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tctraznlium bromide assay. PMNs from healthy volunteers were ineubated with either G-CSF (100-10,000 U/ml) or IFN-γ (10-5000 U/ml) or buffer at 37° C for 90 min and their capacity to damage nonopsonized pseudohyphae was then measured. C. tropicalis appeared to be the most susceptible species, whereas C. parapsilosis showed the highest rate of rcsistancc to PMN damage. G-CSF (500–10T000 U/ml) and IFN-γ (100-1000 U/ml) enhanced the antifungal activity of PMNs against C. albicans pseudo hyphae (P < .01 and P < .05). Among the others, G-CSF enhanced PMN-induccd damage of C. parapsilosis at concentrations 500–10,000 U/ml (P < .05), whereas it enhanced damage of C, tropicalis only at 10,000 U/ml (P < .01), IFN-γ (100–1000 U/ml) primed PMNs also caused augmented damage of C. parapsilosis (P < .05) but not of C, tropicalis at the same concentrations. Species-dependent differences exist in the responses of PMNs to Candida pseudohyphae and G-CSF as well as IFN-γ are important immunomodulators of phagocytic host defenses against them Leukoc. Biol. 57: 651–656; 1995.
Databáze: OpenAIRE
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