The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan
| Autor: | Hilary A. Vaughan, Ian F. C. McKenzie, Vaios Karanikas, Deborah W.M Ho, Mauro S. Sandrin, Geoffrey A. Pietersz |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
T cell
Molecular Sequence Data Cross Reactions Lymphocyte Activation digestive system Macaque Mannans Mice Immune system biology.animal MHC class I medicine Animals Cytotoxic T cell Amino Acid Sequence skin and connective tissue diseases neoplasms MUC1 Mannan Polymorphism Genetic Base Sequence General Veterinary General Immunology and Microbiology biology Mucin-1 Public Health Environmental and Occupational Health Exons Macaca mulatta biological factors digestive system diseases Infectious Diseases medicine.anatomical_structure Tandem Repeat Sequences Immunology biology.protein Molecular Medicine Immunization CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Vaccine. 18:3297-3309 |
| ISSN: | 0264-410X |
| Popis: | Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8(+) cytotoxic T cells and are completely protected from the development of MUC1(+) tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studies in cynomolgus monkeys (Macaca fascicularis), to test the efficacy of mannan-MUC1 in higher primates. Monkey MUC1 genomic clones were isolated from a macaque library, peptides and fusion protein synthesised and mice and monkeys immunised with macaque MUC1-mannan. In mice CTL responses were induced (as has been found with human MUC1 mannan conjugates), but in contrast monkeys produced a humoral response, with no T cell proliferative, cytotoxic responses or CTLp found. In spite of the presence of anti-MUC1 auto-antibodies, there was no toxicity or induction of autoimmunity. |
| Databáze: | OpenAIRE |
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