The Effect of MicroRNA-331-3p on Preadipocytes Proliferation and Differentiation and Fatty Acid Accumulation in Laiwu Pigs
| Autor: | Yongqing Zeng, Jingxiang Cui, Tao Chen, Wei Chen, Lixia Ma |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Article Subject
Swine Cellular differentiation lcsh:Medicine General Biochemistry Genetics and Molecular Biology Mitochondrial Proteins chemistry.chemical_compound microRNA Adipocytes Animals Cells Cultured Cell Proliferation chemistry.chemical_classification Regulation of gene expression General Immunology and Microbiology Fatty acid metabolism Cell growth lcsh:R Fatty Acids Fatty acid Cell Differentiation General Medicine Transfection Metabolism Cell biology MicroRNAs chemistry Gene Expression Regulation Acyltransferases Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2019 (2019) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2019/9287804 |
| Popis: | Objective. The proliferation and differentiation of preadipocytes are regulated by microRNAs (miRNAs), hormones, and other factors. This study aimed to investigate the effects of miR-331-3p on the proliferation and differentiation of preadipocytes in addition to fatty acid metabolism. Methods. Preadipocytes were transfected with miR-331-3p mimics, miR-NC, or miR-331-3p inhibitor to explore its effect on cell proliferation and fatty acid accumulation. Furthermore, preadipocytes were transfected with pre-miR-331-3p, pcDNA3.1(+), or miR-331-3p inhibitor to explore its effect on differentiation. Results. It was observed that miR-331-3p could inhibit preadipocytes proliferation. Furthermore, miR-331-3p was highly expressed during cellular differentiation and appeared to promote the process. In addition, dual fluorescein analysis showed that dihydrolipoamide S-succinyltransferase (DLST) is a target gene of miR-331-3p, and overexpression of miR-331-3p could regulate the metabolism of fatty acids in the citrate pyruvate cycle by targeting DLST expression. Conclusion. In summary, these findings indicated that miR-331-3p exerts contrasting effects on the processes of fat deposition. |
| Databáze: | OpenAIRE |
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