CD163 and CCR7 as markers for macrophage polarisation in lung cancer microenvironment
| Autor: | Piotr Rzepecki, Małgorzata Polubiec-Kownacka, Joanna Domagała-Kulawik, Dariusz Dziedzic, Dominika Wolosz, Iwona Kwiecień |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Immunology
Population Macrophage polarization lcsh:Medicine tgf-1 TGF-β1 medicine Immunology and Allergy Macrophage Lung cancer education education.field_of_study Lung bronchoalveolar lavage fluid medicine.diagnostic_test Chemistry lcsh:R Cancer respiratory system medicine.disease Molecular biology ccr7 respiratory tract diseases m1 macrophages m2 macrophages lung cancer medicine.anatomical_structure Bronchoalveolar lavage Clinical Immunology CD163 |
| Zdroj: | Central European Journal of Immunology, Vol 44, Iss 4, Pp 395-402 (2020) Central-European Journal of Immunology |
| ISSN: | 1644-4124 1426-3912 |
| Popis: | Introduction M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). Aim of the study To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-β. Material and methods Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively). Results We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7lowCD163+), and cells with a stronger CCR7 (CCR7+CD163low). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7lowCD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163low). The median proportion of TGF-β1 concentration was higher in the clBALF and hlBALF supernatant than in the serum. Conclusions In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|