Can Scoliotic Discs Be Controls for Molecular Studies in Intervertebral Disc Research? Insights From Proteomics
Autor: | Sharon Miracle Nayagam, S. Rajasekaran, Chitraa Tangavel, R. Sunmathi, K. S. Sri Vijay Anand, Rishi Mugesh Kanna, Dilip Chand Raja Soundararajan, Ajoy Prasad Shetty, M. Raveendran, B T Pushpa |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
2019-20 coronavirus outbreak Pathology medicine.medical_specialty Coronavirus disease 2019 (COVID-19) medicine.diagnostic_test business.industry Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Intervertebral disc Magnetic resonance imaging medicine.disease Proteomics Degenerative disc disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine Orthopedics and Sports Medicine Surgery Neurology (clinical) business 030217 neurology & neurosurgery |
Zdroj: | Global spine journal. 12(4) |
ISSN: | 2192-5682 |
Popis: | Study Design: Proteomic analysis of human intervertebral discs. Objectives: To compare the characters of scoliotic discs and discs from magnetic resonance imaging (MRI)–normal voluntary organ donors controls used in disc research employing proteomics and establish “true controls” that can be utilized for future intervertebral disc (IVD) research. Methods: Eight MRI-normal discs from 8 brain-dead voluntary organ donors (ND) and 8 scoliotic discs (SD) from 3 patients who underwent anterior surgery for adolescent idiopathic scoliosis were subjected to tandem mass spectrometry, and further analysis was performed. Results: Mass spectrometry identified a total of 235 proteins in ND and 438 proteins in the SD group. Proteins involved in extracellular matrix integrity (Versican, keratins KRT6A, KRT14, KRT5, and KRT 13A1, A-kinase anchor protein 13, coagulation factor XIII A chain, proteoglycan 4) and proteins involved in transcription and DNA repair (Von Willebrand factor A domain-containing 3B, eukaryotic initiation factor 2B, histone H4, leukocyte cell–derived chemotaxin 2) were found to be downregulated in SD. Inflammatory proteins (C3, C1S), and oxidative stress response proteins (peroxiredoxin-2,6, catalase, myeloperoxidase, apolipoprotein E) were found to be upregulated in SD. These changes were reflected at the pathway level also. Conclusion: Findings of our study confirm that scoliotic discs have an abundance of inflammatory, oxidative stress response proteins, which are either absent or downregulated in the ND group indicating that scoliotic discs are not pathologically inert. Furthermore, this study has established MRI-normal discs from voluntary organ donors as the “true” control for molecular studies in IVD research. |
Databáze: | OpenAIRE |
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