The Transmission and Evolution of HIV-1 Quasispecies within One Couple: a Follow-up Study based on Next-Generation Sequencing
| Autor: | Runhua Ye, Qi Zhao, Jibao Wang, Song Duan, Yujie Wen, Yan Jiang, Yu-rong Gong, Feng Kd, Pinliang Pan, Bin Su, Maofeng Qiu, Hao Wu, Fengjiao Yu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Sequence analysis 030106 microbiology lcsh:Medicine HIV Infections Viral quasispecies Biology DNA sequencing Article law.invention Evolution Molecular 03 medical and health sciences law Phylogenetics Humans lcsh:Science Phylogeny Multidisciplinary Phylogenetic tree Sequence Analysis RNA lcsh:R env Gene Products Human Immunodeficiency Virus High-Throughput Nucleotide Sequencing Middle Aged Subtyping Quasispecies 030104 developmental biology Transmission (mechanics) Evolutionary biology HIV-1 lcsh:Q Female Contact tracing Follow-Up Studies |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Next-generation sequencing (NGS) has been successfully used to trace HIV-1 infection. In this study, we investigated the transmission and evolution of HIV-1 quasispecies in a couple infected through heterosexual behavior. A heterosexual couple in which both partners were infected with HIV-1 was followed up for 54 months. Blood samples including whole-blood and plasma samples, were collected at various time points. After HIV-1 subtyping, NGS (Miseq platform) was used to sequence the env region of the HIV-1 quasispecies. Genetic distances were calculated, and phylogenetic trees were generated. We found both partners were infected with HIV-1 subtype circulating recombinant form (CRF), CRF65_cpx. The quasispecies distribution was relatively tightly clustered in the phylogenetic tree during early infection. Over time, the distribution of HIV-1 quasispecies gradually became more dispersed at 12th months, with a progressive increase in gene diversity. By 37th months, the sequences obtained for both partners formed different clusters in the phylogenetic tree. These results suggest that the HIV-1 contact tracing results generated by the Miseq platform may be more reliable than other conventional sequencing methods, which can provide important information about the transmission and evolution of HIV-1. Our findings may help to better target preventative interventions for promoting public health. |
| Databáze: | OpenAIRE |
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