Single-cell RNA-seq reveals functionally distinct biomaterial degradation-related macrophage populations
| Autor: | Yangwu Chen, Yuwei Yang, Boon Chin Heng, Chenqi Tang, Jinchun Ye, Zi Yin, Chunmei Fan, Hong Zhang, Xiao Chen, Yang Fei, Weishan Chen, Weiliang Shen, Hongwei Ouyang, Chengrui An, Hua Liu, Yuanhao Xie, Jiayun Huang |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Cell type Tissue Scaffolds Chemistry Macrophages Cell Biophysics Biomaterial Bioengineering Biocompatible Materials Mechanotransduction Cellular Cell biology Biomaterials medicine.anatomical_structure Tissue engineering Mechanics of Materials In vivo Ceramics and Composites medicine Macrophage RNA-Seq Mechanotransduction |
| Zdroj: | Biomaterials. 277 |
| ISSN: | 1878-5905 |
| Popis: | Macrophages play crucial roles in host tissue reaction to biomaterials upon implantation in vivo. However, the complexity of biomaterial degradation-related macrophage subpopulations that accumulate around the implanted biomaterials in situ is not fully understood. Here, using single cell RNA-seq, we analyze the transcriptome profiles of the various cell types around the scaffold to map the scaffold-induced reaction, in an unbiased approach. This enables mapping of all biomaterial degradation-associated cells at high resolution, revealing distinct subpopulations of tissue-resident macrophages as the major cellular sources of biomaterial degradation in situ. We also find that scaffold architecture can affect the mechanotransduction and catabolic activity of specific material degradation-related macrophage subpopulations in an Itgav-Mapk1-Stat3 dependent manner, eventually leading to differences in scaffold degradation rate in vivo. Our work dissects unanticipated aspects of the cellular and molecular basis of biomaterial degradation at the single-cell level, and provides a conceptual framework for developing functional tissue engineering scaffolds in future. |
| Databáze: | OpenAIRE |
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