BRAF analysis on a spectrum of melanocytic neoplasms: an epidemiological study across differing UV regions
| Autor: | Ibrahim Khalifeh, Christian Oberkanins, Suad Taraif, Salwa S. Sheikh, Sarah Karram, Robert H. Habib, Mohammad Adib Houreih, Mohamed Satti, Samir S. Amr, Maya Saroufim, Asif Loya, Cleo Youssef Massad |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Oncology
Adult Male Proto-Oncogene Proteins B-raf Mutation rate medicine.medical_specialty Pathology Skin Neoplasms Adolescent Ultraviolet Rays DNA Mutational Analysis Dermatology Biology Pathology and Forensic Medicine Young Adult Internal medicine Epidemiology medicine Mutation type Humans Child neoplasms Melanoma Nevus Aged Aged 80 and over integumentary system Reverse Transcriptase Polymerase Chain Reaction Incidence (epidemiology) Incidence Infant General Medicine Middle Aged medicine.disease Child Preschool Cohort Mutation (genetic algorithm) Sunlight Female V600E |
| Zdroj: | The American Journal of dermatopathology. 36(1) |
| ISSN: | 1533-0311 |
| Popis: | BRAF mutation has been linked to the development of melanocytic tumors in homogeneous Caucasian cohorts. The role of solar UV radiation (UVR) in BRAF mutation status is poorly understood. We studied the epidemiology of BRAF mutation across a spectrum of melanocytic neoplasms in populations with differing UVR rates. Extended testing for 9 mutation types was attempted on 600 melanocytic neoplasms including banal nevi (n = 225), dys- plastic nevi (n = 113), primary (n = 172), and metastatic melano- mas (n = 90). Specimens were collected from 4 countries with increasing UVR rates (in kJ/m 2 /yr): Syria (n = 45; UVR = 93.5), Lebanon (n = 225; UVR = 110), Pakistan (n = 122; UVR = 128), and Saudi Arabia (n = 208; UVR = 139). UVR was estimated from 21-year averages from The National Center for Atmospheric Research database. The overall BRAF mutation rate was 49% (268 of 545) and differed significantly by the geographic location (34% Pakistan, 49% Lebanon, 67% Syria, and 54% Saudi Arabia; P = 0.001), neoplasm type (P , 0.001), and anatomical location (P , 0.001) but not with age (P = 0.07) and gender (P = 1.0). V600E was the predominant mutation type, found in 96.3% of the cases. Incidence of melanoma was significantly greater in BRAF- negative (39%) versus BRAF-positive (17%) groups. For BRAF- positive cases, less severe lesions were systematically more frequent (P , 0.001). Multivariate analysis indicated that BRAF mutation is predicted by neoplasm type, anatomical site, and geographic location. In our Near East cohort, BRAF mutation rates varied by geographic location but not based on UVR. BRAF-positive status was associated with less severe lesions. |
| Databáze: | OpenAIRE |
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