Enhancing the chondrogenic potential of chondrogenic progenitor cells by deleting RAB5C
| Autor: | Elvira Henze, Ruth Neumann, Jerome Nicolas Janssen, Valerio Izzi, Vicki Rosen, Gökhan Cingöz, Florian Lowen, Christof Lenz, David Küttner, Nicolai Miosge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Proteomics Science Omics 02 engineering and technology SOX9 Osteoarthritis Matrix (biology) Article 03 medical and health sciences medicine Progenitor cell Transcriptomics Gene knockdown Multidisciplinary Chemistry Cartilage 021001 nanoscience & nanotechnology Chondrogenesis medicine.disease 3. Good health Cell biology RUNX2 030104 developmental biology medicine.anatomical_structure Stem cells research 0210 nano-technology |
| Zdroj: | iScience iScience, Vol 24, Iss 5, Pp 102464-(2021) |
| Popis: | Summary Osteoarthritis (OA) is the most prevalent chronic joint disease that affects a large proportion of the elderly population. Chondrogenic progenitor cells (CPCs) reside in late-stage OA cartilage tissue, producing a fibrocartilaginous extracellular matrix; these cells can be manipulated in vitro to deposit proteins of healthy articular cartilage. CPCs are under the control of SOX9 and RUNX2. In our earlier studies, we showed that a knockdown of RUNX2 enhanced the chondrogenic potential of CPCs. Here we demonstrate that CPCs carrying a knockout of RAB5C, a protein involved in endosomal trafficking, exhibited elevated expression of multiple chondrogenic markers, including the SOX trio, and increased COL2 deposition, whereas no changes in COL1 deposition were observed. We report RAB5C as an attractive target for future therapeutic approaches designed to increase the COL2 content in the diseased joint. Graphical abstract Highlights • RUNX2 has a pro-chondrogenic effect in CPCs. • RAB5C is a potential interaction partner of SOX9 in CPCs. • Loss of RAB5C increases the chondrogenic potential of CPCs. Stem cells research; Omics; Proteomics ; Transcriptomics |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|