Negative regulation of hematopoiesis by the fused in myeloproliferative disorders gene product
| Autor: | Keisuke Okita, Tetsuya Taga, Ikuo Nobuhisa, Marie Josèphe Pébusque, Hirofumi Inoue, Makiko Takizawa |
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| Rok vydání: | 2004 |
| Předmět: |
Recombinant Fusion Proteins
Biophysics Gene Expression Stem cell factor Biology Biochemistry Gene product Mice Myeloproliferative Disorders Proto-Oncogene Proteins Cell Adhesion Animals Humans Receptor Fibroblast Growth Factor Type 1 Gonads Molecular Biology Aorta Cells Cultured Fibroblast growth factor receptor 1 Gene Expression Regulation Developmental Receptor Protein-Tyrosine Kinases Cell Biology Receptors Fibroblast Growth Factor Fusion protein Embryonic stem cell Hematopoiesis Cell biology DNA-Binding Proteins GATA2 Transcription Factor Core Binding Factor Alpha 2 Subunit Immunology Stem cell human activities Tyrosine kinase Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 313:125-128 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2003.11.097 |
| Popis: | The t(8;13) translocation, found in a rare and aggressive type of stem cell myeloproliferative disorder, leads to the generation of a fusion protein between the N-terminal gene product of fused in myeloproliferative disorders (FIM)/ZNF198 and the fibroblast growth factor receptor 1 (FGFR1) kinase domain. The chimeric protein was reported to have constitutively activated tyrosine kinase activity. However, little is known about a role of FIM in hematopoietic cell regulation. Here we show that FIM protein is ubiquitously expressed in mouse embryonic tissues but much less in hematopoietic cells. We also show that forced expression of FIM inhibits the emergence of hematopoietic cells in the cultured mouse aorta-gonad-mesonephros (AGM) region on embryonic day (E) 11.5, where definitive hematopoiesis is first found during embryogenesis. These results suggest that the expression level of FIM determines the development of hematopoiesis during mouse ontogeny. |
| Databáze: | OpenAIRE |
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