Initial ontogeny of digestive enzymes in the early life stages of captive-bred European eels during fasting: A partial characterization
| Autor: | Monica Forni, Pietro Emmanuele, Irvin Tubon, Albamaria Parmeggiani, Augusta Zannoni, Antonio Casalini, Oliviero Mordenti |
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| Přispěvatelé: | Parmeggiani A., Zannoni A., Tubon I., Casalini A., Emmanuele P., Forni M., Mordenti O. |
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
animal structures 040301 veterinary sciences Ontogeny Zoology Aquaculture 0403 veterinary science 03 medical and health sciences Critically endangered Digestive System Physiological Phenomena medicine Animals Trypsin Amylase Larval culture 030304 developmental biology Cholecystokinin 0303 health sciences Larva General Veterinary biology Hatching 04 agricultural and veterinary sciences Lipase Digestive enzyme Anguilla European eel Amylases biology.protein Animal Nutritional Physiological Phenomena Female Gene expression Food Deprivation medicine.drug |
| Zdroj: | Research in veterinary science. 132 |
| ISSN: | 1532-2661 |
| Popis: | The European eel has recently been included on the Red List of the International Union for Conservation of Nature (IUCN) as a critically endangered species. The rearing of Anguilla larvae is seen as a key bottleneck to the mass production of glass eels since very little ecological information is available regarding their natural nutrition. Studies of digestive physiology and ontogenetic development in eel larvae could provide useful information for solving some of the puzzles regarding larval fish culture. The aim of this study was to characterize the ontogeny of pancreatic enzymes (trypsin, lipase and amylase) and a peptide hormone regulator of pancreatic secretion (cholecystokinin) in terms of gene expression in European eel larvae from day 0 (P0) of hatching to 5, 10, 15 and 20 days post hatching during fasting. The results in the present study showed that all the genes selected were present, with different levels of expression and increasing trends, during larval development. At P0, the increase in the gene expression of lipase and amylase was higher than that of trypsin and cholecystokinin, confirming that enzymatic activity began before mouth opening and that larvae, provided with a complete enzymatic set, might have the capacity of digesting and absorbing various nutrients. |
| Databáze: | OpenAIRE |
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