Novel glass slide preparation system for single DNA molecules analysis
| Autor: | Marina N. Nedelcheva-Veleva, Alexander Zarkov, Stoyno S. Stoynov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
DNA stretching glass slide surface modification engineering.material Bioinformatics medicine.disease_cause chemistry.chemical_compound single DNA molecules Coating chemistry Polymerization Chemical engineering Acrylic monomers Glass slide engineering Fluorescence microscope medicine Molecule DNA Ultraviolet Articles Medical Biotechnology Biotechnology fluorescent microscopy |
| Zdroj: | Biotechnology, Biotechnological Equipment |
| ISSN: | 1314-3530 1310-2818 |
| Popis: | Here we propose an easy to build up and apply glass slide preparation system for single DNA molecules stretching. It is based on fast and simple coating of a solid glass with a cocktail of acrylic monomers that are easily polymerized via ultraviolet illumination. The acrylated slides are used to successfully stretch DNA molecules in a broader pH range compared to that of the commonly used amino-silanes. Moreover, the single DNA molecules that are stretched on the acrylated slides give a brighter and more photostable signal when visualized under a fluorescent microscope. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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