Association of NRG3 and ERBB4 gene polymorphism with nicotine dependence in Turkish population
| Autor: | Şenay Görücü Yılmaz, Kamuran Karakülah, Hale Güler Kara, Ceyhan Balcı Şengül, Mehmet Emin Erdal, Cem Şengül |
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| Rok vydání: | 2021 |
| Předmět: |
Nicotine dependence
Target Risk Adult Male medicine.medical_specialty Turkish population Nicotine Receptor ErbB-4 Smoking-Cessation Genotype Turkey Addiction Single-nucleotide polymorphism Expression Polymorphism Single Nucleotide Neuregulin 3 Internal medicine Genetics medicine Humans Genetic Predisposition to Disease Polymorphism Molecular Biology ERBB4 Neuregulins business.industry Smoking General Medicine DNA Tobacco Use Disorder Middle Aged medicine.disease NRG3 Nicotine withdrawal Endocrinology Population study Anxiety Female Gene polymorphism medicine.symptom business Alcohol medicine.drug |
| Zdroj: | Molecular biology reports. 48(6) |
| ISSN: | 1573-4978 |
| Popis: | Background Nicotine dependence (ND) is characterized by regular smoking, anxiety, irritation, difficulty concentrating, impatience, restlessness, tremor, dizziness, hunger, nicotine demand, and the individual's reluctance to quit despite knowing the health risks of smoking. Recently, it has been reported that the Neuregulin 3 (NRG3)/Erb-B2 receptor tyrosine kinase 4 (ERBB4) signaling pathway plays a role in ND. NRG3, which is activated after nicotine intake, binds to ERBB4 and causes GABA release. GABA reduces anxiety and tension, which are one of the nicotine withdrawal symptoms. Therefore we aimed to investigate the relationship between NRG3 and ERBB4 gene polymorphisms and ND. Materials and methods The study population was comprised of patients with ND (n = 200) and healthy non-smoker control subjects (n = 200) who were matched for age, sex, and compared for comorbidity factors such as alcohol, smoking, duration, and education (age range 18-60). Genotypes were detected by Real-Time PCR using TaqMan technology. The Fagerstrom Nicotine Dependence Test (FTND) score was 5 and above for the patient group and 0 for the control group. DNA was obtained from whole peripheral blood and six polymorphisms of Neuregulin 3 (NRG3) (rs1836724, rs7562566, and rs10048757) and Erb-B2 Receptor Tyrosine Kinase 4 (ERBB4) (rs1764072, rs6584400, and rs10883934) genes were analyzed by real-time PCR method. Results Our findings show that the six selected SNPs are not significantly associated with ND in the Turkish population and no correlation with dependence levels (p > 0.05). Conclusion Although our findings do not show a relationship between ND and these polymorphisms, it is the first study to investigate these single nucleotide polymorphisms (SNPs) for the first time in ND and to find some genotypes in the Turkish population when compared to other populations. Also, our findings are important in terms of their contribution to the literature and forensic genetics. Green Crescent and Mersin University Scientific Research Project Unit [BAP-SBE TBB (HG) 2014-4 YL] We would like to thank Green Crescent and Mersin University Scientific Research Project Unit for supporting this study as a project coded BAP-SBE TBB (HG) 2014-4 YL. |
| Databáze: | OpenAIRE |
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