Comparison of tracheal and choanal cleft swabs and poultry dust samples for detection of Newcastle disease virus and infectious bronchitis virus genome in vaccinated meat chicken flocks
| Autor: | Stephen W. Walkden-Brown, Sheridan Alfirevich, Priscilla F. Gerber, Mark Stillman, Awol M. Assen |
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| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Veterinary medicine animal diseases viruses Infectious bronchitis virus Pathology and Laboratory Medicine Bird Genomics Genome Poultry 0403 veterinary science Medical Conditions Medicine and Health Sciences Gamefowl Materials 0303 health sciences Vaccines Multidisciplinary Vaccination Eukaryota Dust 04 agricultural and veterinary sciences Genomics Viral Load Infectious Diseases Medical Microbiology Viral Pathogens embryonic structures Vertebrates Viruses Physical Sciences Medicine Pathogens Coronavirus Infections Viral load Research Article animal structures Infectious Disease Control 040301 veterinary sciences Science Newcastle Disease Materials Science Newcastle disease virus Genome Viral Biology Newcastle disease Microbiology Virus Birds 03 medical and health sciences Virology Genetics Animals Microbial Pathogens Poultry Diseases 030304 developmental biology Organisms Biology and Life Sciences Viral Vaccines biology.organism_classification Meat chicken Animal Genomics Fowl Amniotes Paramyxoviruses Flock Chickens Zoology Viral Transmission and Infection |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 4, p e0247729 (2021) |
| ISSN: | 1932-6203 |
| Popis: | This study assessed different methods (tracheal and choanal cleft swabs from individual birds, and poultry dust as a population level measure) to evaluate the shedding kinetics of infectious bronchitis virus (IBV) and Newcastle disease virus (NDV) genome in meat chicken flocks after spray vaccination at hatchery. Dust samples and tracheal and choanal cleft swabs were collected from four meat chicken flocks at 10, 14, 21 and 31 days post vaccination (dpv) and tested for IBV and NDV genome copies (GC) by reverse transcriptase (RT)-PCR. IBV and NDV GC were detected in all sample types throughout the study period. Detection rates for choanal cleft and tracheal swabs were comparable, with moderate and fair agreement between sample types for IBV (McNemar’s = 0.27, kappa = 0.44) and NDV (McNemar’s = 0.09; kappa = 0.31) GC respectively. There was no significant association for IBV GC in swabs and dust samples (R2 = 0.15, P = 0.13) but NDV detection rates and viral load in swabs were strongly associated with NDV GC in dust samples (R2 = 0.86 and R2 = 0.90, P |
| Databáze: | OpenAIRE |
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