Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease
| Autor: | Tamás Visnovitz, Károly Vékey, Lilla Turiák, Éva Pállinger, Barbara W Sódar, Edit I. Buzás, Gabriella Pocsfalvi, Árpád Kovács |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Quality Control Databases Factual Centrifugation Proteomics Biochemistry Workflow 03 medical and health sciences proteomics Extracellular Humans Biomarker discovery Molecular Biology mass spectrometry Chemistry Computational Biology Membrane Proteins Extracellular vesicle Microvesicles Cell biology Body Fluids 030104 developmental biology Culture Media Conditioned Nucleic acid Biomarker (medicine) Ultracentrifuge extracellular vesicles Biomarkers Software Chromatography Liquid |
| Zdroj: | Expert review of proteomics (2017): 1–18. doi:10.1080/14789450.2017.1392244 info:cnr-pdr/source/autori:Sodar, Barbara W; Kovacs, Arpad; Visnovitz, Tamas; Pallinger, Eva; Vekey, Karoly; Pocsfalvi, Gabriella; Turiak, Lilla; Buzas, Edit/titolo:Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease./doi:10.1080%2F14789450.2017.1392244/rivista:Expert review of proteomics (Print)/anno:2017/pagina_da:1/pagina_a:18/intervallo_pagine:1–18/volume |
| DOI: | 10.1080/14789450.2017.1392244 |
| Popis: | INTRODUCTION: Extracellular vesicles are emerging sources of biomarkers for modern preventive and precision medicine. Extracellular vesicles in body fluids offer a unique opportunity for integrative biomarker approaches due to their complex biocargo that includes proteins, lipids, nucleic acids and metabolites. Mass spectrometry-based proteomics data suggest that a significant portion of human proteins are sorted into extracellular vesicles and amenable for biomarker discovery schemes. Areas covered: this review focuses on key aspects of isolation, quality control and subsequent analysis of blood plasma- and conditioned medium-derived extracellular vesicle proteins, and summarizes the current state-of-the-art in the field. Furthermore, it provides introduction and guidelines for mass spectrometry-based proteomic analysis of extracellular vesicles. Expert commentary: Comparison of newly developed isolation and purification techniques with classical ultracentrifugation-based approaches are highly recommended. It is also essential to use multiple analytical approaches to characterize the isolated extracellular vesicles prior to characterization of their biocargo. Rigor in data reproducibility, critical data analysis, awareness of potential pitfalls, standardization and benchmarking are required for extracellular vesicle research to fulfil the current expectation that these subcellular structures can become a valid source of next generation biomarkers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|