Comparative Genomics Revealed Multiple Helicobacter pylori Genes Associated with Biofilm Formation In Vitro
| Autor: | Fanny Peters, Khean-Lee Goh, Barry J. Marshall, Mun Fai Loke, Alfred Tay, Chow Goon Ng, Bow Ho, Jamuna Vadivelu, Eric Hong Jian Wong, Eng Guan Chua |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Membranes lcsh:Medicine Pathogenesis medicine.disease_cause Pathology and Laboratory Medicine Helicobacter Medicine and Health Sciences lcsh:Science Genetics Multidisciplinary Ecology High-Throughput Nucleotide Sequencing Genomics Bacterial Pathogens Medical Microbiology Pathogens Cellular Structures and Organelles Research Article Pathogen Motility Sequence analysis Virulence Factors 030106 microbiology Biology In Vitro Techniques Microbiology Ecosystems 03 medical and health sciences Microbial Ecosystems Bacterial Proteins Microbial Control medicine CagA Escherichia coli Microbial Pathogens Comparative genomics Pharmacology Bacteria Helicobacter pylori lcsh:R Ecology and Environmental Sciences Biofilm Organisms Biology and Life Sciences Membrane Proteins Bacteriology Cell Biology Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification Outer Membrane Proteins Pathogenicity island Streptococcus mutans 030104 developmental biology Biofilms Antibiotic Resistance lcsh:Q Antimicrobial Resistance Bacterial Biofilms Genome Bacterial |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0166835 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Background Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. Materials and Methods Biofilm yield of 32 Helicobacter pylori strains (standard strain and 31 clinical strains) were determined by crystal-violet assay and grouped into poor, moderate and good biofilm forming groups. Whole genome sequencing of these 32 clinical strains was performed on the Illumina MiSeq platform. Annotation and comparison of the differences between the genomic sequences were carried out using RAST (Rapid Annotation using Subsystem Technology) and SEED viewer. Genes identified were confirmed using PCR. Results Genes identified to be associated with biofilm formation in H. pylori includes alpha (1,3)-fucosyltransferase, flagellar protein, 3 hypothetical proteins, outer membrane protein and a cag pathogenicity island protein. These genes play a role in bacterial motility, lipopolysaccharide (LPS) synthesis, Lewis antigen synthesis, adhesion and/or the type-IV secretion system (T4SS). Deletion of cagA and cagPAI confirmed that CagA and T4SS were involved in H. pylori biofilm formation. Conclusions Results from this study suggest that biofilm formation in H. pylori might be genetically determined and might be influenced by multiple genes. Good, moderate and poor biofilm forming strain might differ during the initiation of biofilm formation. |
| Databáze: | OpenAIRE |
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