Nucleo-plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis

Autor: Maha Chieb, Kevin Pounot, Pierre Gans, Fabien Chevalier, Louise Chambon, Thomas Pfannschmidt, Adrien Favier, Robert Blanvillain, Monique Liebers, François-Xavier Gillet, abir Israel, David Cobessi, Remi Ruedas, Elisabetta Boeri Erba
Přispěvatelé: Dynamiques Chromatiniennes et Transitions Développementales (ChromDev), Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Institut de biologie structurale (IBS - UMR 5075), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), ANR-17-CE11-0031,PEPRegulChloro3D,Biogenèse du chloroplaste: fonction, régulation et structure de l'ARN-polymérase plastidiale et de ses protéines associées PAP(2017), European Project: AGIR
Rok vydání: 2020
Předmět:
0106 biological sciences
Chloroplasts
Light
Transcription
Genetic
Mutant
Arabidopsis
01 natural sciences
chemistry.chemical_compound
0302 clinical medicine
Gene Expression Regulation
Plant
RNA polymerase
Gene expression
Morphogenesis
Arabidopsis thaliana
Plastids
photobodies
0303 health sciences
Organelle Biogenesis
Phytochrome
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
General Neuroscience
food and beverages
DNA-Directed RNA Polymerases
Articles
Plants
Genetically Modified
3. Good health
Cell biology
Chloroplast
Photomorphogenesis
Arabidopsis PEP/PAPs
Signal Transduction
Nuclear gene
Acid Phosphatase
education
Biology
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chloroplast
Plastid
Molecular Biology
030304 developmental biology
Cell Nucleus
General Immunology and Microbiology
Arabidopsis Proteins
fungi
biology.organism_classification
biogenesis
photomorphogenesis
chemistry
030217 neurology & neurosurgery
Biogenesis
Transcription Factors
010606 plant biology & botany
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 2020, 39 (22), pp.e104941. ⟨10.15252/embj.2020104941⟩
EMBO Journal, 2020, 39 (22), pp.e104941. ⟨10.15252/embj.2020104941⟩
EMBO J
ISSN: 0261-4189
1460-2075
DOI: 10.1101/2020.03.10.985002
Popis: International audience; The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi-autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid-encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome-mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre-sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B-mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP-driven chloroplastic gene expression during chloroplast biogenesis.
Databáze: OpenAIRE
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